Sindiani Amer Mahmoud, Batiha Osamah, Al-Zoubi Esra'a, Khadrawi Sara, Alsoukhni Ghadeer, Alkofahi Ayesha, Alahmad Nour Alhoda, Shaaban Sherin, Alshdaifat Eman, Abu-Halima Masood
Department of Obstetrics and Gynecology, Faculty of Medicine, Jordan University of Science and Technology, Irbid, Jordan.
Department of Biotechnology and Genetic Engineering, Jordan University of Science and Technology, Irbid, Jordan.
Clin Exp Reprod Med. 2021 Mar;48(1):69-79. doi: 10.5653/cerm.2020.03706. Epub 2021 Jan 28.
Poor ovarian response (POR) refers to a subnormal follicular response that leads to a decrease in the quality and quantity of the eggs retrieved after ovarian stimulation during assisted reproductive treatment (ART). The present study investigated the associations of multiple variants of the estrogen receptor 2 (ESR2) and follicle-stimulating hormone receptor (FSHR) genes with POR in infertile Jordanian women undergoing ART.
Four polymorphisms, namely ESR2 rs1256049, ESR2 rs4986938, FSHR rs6165, and FSHR rs6166, were investigated in 60 infertile Jordanian women undergoing ART (the case group) and 60 age-matched fertile women (the control group), with a mean age of 33.60±6.34 years. Single-nucleotide polymorphisms (SNPs) were detected by restriction fragment length polymorphism and then validated using Sanger sequencing.
The p-value of the difference between the case and control groups regarding FSHR rs6166 was very close to 0.05 (p=0.054). However, no significant differences were observed between the two groups in terms of the other three SNPs, namely ESR2 rs1256049, ESR2 rs4986938, and FSHR rs6165 (p=0.561, p=0.433, and p=0.696, respectively).
The association between FSHR rs6166 and POR was not statistically meaningful in the present study, but the near-significant result of this experiment suggests that statistical significance might be found in a future study with a larger number of patients.
卵巢反应不良（POR）是指卵泡反应异常，导致辅助生殖治疗（ART）过程中卵巢刺激后获取的卵子质量和数量下降。本研究调查雌激素受体2（ESR2）和促卵泡激素受体（FSHR）基因的多个变体与接受ART的约旦不孕女性POR之间的关联。
对60名接受ART的约旦不孕女性（病例组）和60名年龄匹配的有生育能力的女性（对照组）进行了四项多态性研究，即ESR2 rs1256049、ESR2 rs4986938、FSHR rs6165和FSHR rs6166，平均年龄为33.60±6.34岁。通过限制性片段长度多态性检测单核苷酸多态性（SNP），然后使用桑格测序进行验证。
病例组和对照组在FSHR rs6166方面的差异p值非常接近0.05（p = 0.054）。然而，在其他三个SNP方面，两组之间未观察到显著差异，即ESR2 rs1256049、ESR2 rs4986938和FSHR rs6165（分别为p = 0.561、p = 0.433和p = 0.696）。
在本研究中，FSHR rs6166与POR之间的关联在统计学上无意义，但该实验接近显著的结果表明，在未来更大样本量患者的研究中可能会发现统计学意义。
