Ryder M I, Weinreb R N, Niederman R
Department of Stomatology, University of California, San Francisco 94143.
Anat Rec. 1988 Jul;221(3):679-86. doi: 10.1002/ar.1092210302.
We examined the relationship of microtubules to the granule organization in stimulated human polymorphonuclear leukocytes (PMNs). Electron microscopic (EM) observations of critical-point-dried PMNs revealed that only a portion of the granules appeared in close association to microtubules. These closely associated granules appeared to be attached to the microtubule via smaller-diameter filaments. The remaining granules appeared either attached to microtubules at a further distance, via smaller-diameter filaments such as actin, or unassociated with microtubules. EM observations of PMNs treated with either the microtubule promoter drug taxol or the microtubule depolymerization drugs nocodozole and colchicine revealed a redistribution of granules towards the nucleus. Granule clustering at the periphery of the cell was also noted with nocodozole and colchicine. With cytochalasin B, a uniform distribution of granules was noted. However, granule clustering was noted when PMNs were coincubated with cytochalasin B and colchicine. These results indicate that microtubules may have both a direct and indirect role (through other cytoskeletal elements) in the organization of PMN granules.
我们研究了微管与受刺激的人多形核白细胞（PMN）中颗粒组织的关系。对临界点干燥的PMN进行电子显微镜（EM）观察发现，只有一部分颗粒似乎与微管紧密相关。这些紧密相关的颗粒似乎通过直径较小的细丝附着在微管上。其余的颗粒要么通过肌动蛋白等直径较小的细丝在更远的距离附着在微管上，要么与微管不相关。对用微管促进药物紫杉醇或微管解聚药物诺考达唑和秋水仙碱处理的PMN进行EM观察发现，颗粒向细胞核重新分布。用诺考达唑和秋水仙碱处理时，还注意到细胞周边的颗粒聚集。用细胞松弛素B处理时，注意到颗粒均匀分布。然而，当PMN与细胞松弛素B和秋水仙碱共同孵育时，注意到颗粒聚集。这些结果表明，微管可能在PMN颗粒的组织中具有直接和间接作用（通过其他细胞骨架成分）。
