电穿孔诱导产气荚膜梭菌完整细胞的转化
Electroporation-induced transformation of intact cells of Clostridium perfringens.
作者信息
Allen S P, Blaschek H P
机构信息
Department of Food Science, University of Illinois, Urbana 61801.
出版信息
Appl Environ Microbiol. 1988 Sep;54(9):2322-4. doi: 10.1128/aem.54.9.2322-2324.1988.
Abstract
Electroporation-induced transformation of intact cells of Clostridium perfringens 3624A with plasmids pAMB1 and pHR106 resulted in 3.8 X 10(-5) and 4.2 X 10(-4) transformants per viable cell, respectively. With respect to shuttle plasmid pHR106, these values represent a greater than 100-fold increase in transformation frequency when compared with the values reported with polyethylene glycol-induced L-phase variants.
摘要
用质粒pAMB1和pHR106对产气荚膜梭菌3624A的完整细胞进行电穿孔诱导转化，分别产生了每存活细胞3.8×10⁻⁵和4.2×10⁻⁴个转化体。对于穿梭质粒pHR106，与聚乙二醇诱导的L期变体报道的值相比，这些值代表转化频率提高了100倍以上。
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