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一种源自七鳃鳗超神经体组织的新型蛋白，对肿瘤细胞具有高效细胞毒性作用。

A novel protein derived from lamprey supraneural body tissue with efficient cytocidal actions against tumor cells.

机构信息

College of Life Science, Liaoning Normal University, Dalian, 116081, China.

Lamprey Research Center, Liaoning Normal University, Dalian, 116081, China.

出版信息

Cell Commun Signal. 2017 Oct 16;15(1):42. doi: 10.1186/s12964-017-0198-6.

DOI:10.1186/s12964-017-0198-6
PMID:29037260
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5644163/
Abstract

BACKGROUND

In previous research, we found that cell secretion from the adult lamprey supraneural body tissues possesses cytocidal activity against tumor cells, but the protein with cytocidal activity was unidentified.

METHODS

A novel lamprey immune protein (LIP) as defense molecule was first purified and identified in jawless vertebrates (cyclostomes) using hydroxyapatite column and Q Sepharose Fast Flow column. After LIP stimulation, morphological changes of tumor cells were analysed and measured whether in vivo or in vitro.

RESULTS

LIP induces remarkable morphological changes in tumor cells, including cell blebbing, cytoskeletal alterations, mitochondrial fragmentation and endoplasmic reticulum vacuolation, and most of the cytoplasmic and organelle proteins are released following treatment with LIP. LIP evokes an elevation of intracellular calcium and inflammatory molecule levels. Our analysis of the cytotoxic mechanism suggests that LIP can upregulate the expression of caspase 1, RIPK1, RIP3 to trigger pyroptosis and necroptosis. To examine the effect of LIP in vivo, tumor xenograft experiments were performed, and the results indicated that LIP inhibits tumor growth without damage to mice. In addition, the cytotoxic action of LIP depended on the phosphatidylserine (PS) content of the cell membrane.

CONCLUSIONS

These observations suggest that LIP plays a crucial role in tumor cell survival and growth. The findings will also help to elucidate the mechanisms of host defense in lamprey.

摘要

背景

在之前的研究中，我们发现来自成体七鳃鳗神经上体组织的细胞分泌具有细胞毒性活性的物质，可以杀死肿瘤细胞，但具有细胞毒性活性的蛋白质尚未鉴定。

方法

使用羟基磷灰石柱和 Q Sepharose Fast Flow 柱首次从无颌脊椎动物（圆口类）中分离和鉴定了一种新型七鳃鳗免疫蛋白（LIP）作为防御分子。在体外和体内研究了 LIP 刺激后肿瘤细胞的形态变化。

结果

LIP 诱导肿瘤细胞发生明显的形态变化，包括细胞起泡、细胞骨架改变、线粒体碎片化和内质网空泡化，并且在用 LIP 处理后，大部分细胞质和细胞器蛋白被释放。LIP 引起细胞内钙和炎症分子水平的升高。我们对细胞毒性机制的分析表明，LIP 可以上调半胱天冬酶 1、RIPK1、RIP3 的表达，从而引发细胞焦亡和坏死性凋亡。为了研究 LIP 在体内的作用，进行了肿瘤异种移植实验，结果表明 LIP 抑制肿瘤生长而不会损害小鼠。此外，LIP 的细胞毒性作用取决于细胞膜上的磷脂酰丝氨酸（PS）含量。

结论

这些观察结果表明，LIP 在肿瘤细胞的存活和生长中发挥着关键作用。这些发现也将有助于阐明七鳃鳗宿主防御的机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3002/5644163/93c2ae363308/12964_2017_198_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3002/5644163/274e0d9f01a0/12964_2017_198_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3002/5644163/73364f80a15b/12964_2017_198_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3002/5644163/f648cf4b686e/12964_2017_198_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3002/5644163/14b32e92b0f0/12964_2017_198_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3002/5644163/34a099167b43/12964_2017_198_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3002/5644163/9c5f6304f8cb/12964_2017_198_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3002/5644163/d3727cdaf48e/12964_2017_198_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3002/5644163/93c2ae363308/12964_2017_198_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3002/5644163/274e0d9f01a0/12964_2017_198_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3002/5644163/73364f80a15b/12964_2017_198_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3002/5644163/f648cf4b686e/12964_2017_198_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3002/5644163/14b32e92b0f0/12964_2017_198_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3002/5644163/34a099167b43/12964_2017_198_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3002/5644163/9c5f6304f8cb/12964_2017_198_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3002/5644163/d3727cdaf48e/12964_2017_198_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3002/5644163/93c2ae363308/12964_2017_198_Fig8_HTML.jpg

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本文引用的文献

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2
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Springerplus. 2015 Oct 1;4:569. doi: 10.1186/s40064-015-1270-6. eCollection 2015.
3
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Cell Commun Signal. 2022 Aug 17;20(1):124. doi: 10.1186/s12964-022-00933-0.
4
Molecular Evolution of Transforming Growth Factor-β (TGF-β) Gene Family and the Functional Characterization of Lamprey TGF-β2.转化生长因子-β（TGF-β）基因家族的分子进化及七鳃鳗TGF-β2的功能特性
Front Immunol. 2022 Mar 4;13:836226. doi: 10.3389/fimmu.2022.836226. eCollection 2022.
5
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Toxins (Basel). 2021 Jul 31;13(8):538. doi: 10.3390/toxins13080538.
6
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7
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