College of Life Sciences, Liaoning Normal University, Dalian, 116081, China; Lamprey Research Center, Liaoning Normal University, Dalian, 116081, China.
College of Life Sciences, Liaoning Normal University, Dalian, 116081, China; Lamprey Research Center, Liaoning Normal University, Dalian, 116081, China; Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian, 116023, China.
Mol Immunol. 2020 Aug;124:25-34. doi: 10.1016/j.molimm.2020.05.015. Epub 2020 Jun 1.
Tumor necrosis factor receptor superfamily (TNFRSF) is an ancient protein superfamily. By binding to tumor necrosis factor (TNF), it can participate in inflammatory response, apoptosis, lymphocyte homeostasis and tissue development. Seven TNFR members have previously been identified in lampreys but detailed functions of TNFR members are not yet to be resolved. Here, we demonstrate some of the distinguishing features of TNFR10-like member which belongs to TNFRSF. The immunohistochemical results indicate that the TNFR10-like protein is abundant in vascular epithelial cells of the lamprey typhlosole and gills. The expression of tnfr10-like gene has a significantly increased at transcription level after Vibrio anguillarum, Staphylococcus aureus and Poly (I:C) stimulation. Notably, TNFR10-like is specifically expressed in the granulocytes of lamprey peripheral blood and supraneural body. Besides, overexpression tnfr10-like gene in HEK-293 T cells cause a decrease in cell activity and able to activate nuclear transcription factor-κB (NF-κB). Together, these results imply that L-TNFR10-like may play a vital role as a potential marker in lamprey granulocytes and may also be involved in regulation of immune response mediated by itself.
肿瘤坏死因子受体超家族（TNFRSF）是一个古老的蛋白质超家族。通过与肿瘤坏死因子（TNF）结合，它可以参与炎症反应、细胞凋亡、淋巴细胞稳态和组织发育。此前在七鳃鳗中已鉴定出 7 种 TNFR 成员，但 TNFR 成员的详细功能尚待解决。在这里，我们展示了属于 TNFRSF 的 TNFR10 样成员的一些特征。免疫组织化学结果表明，TNFR10 样蛋白在七鳃鳗的背鳍和鳃中的血管上皮细胞中丰富表达。tnfr10 样基因的表达在受到鳗弧菌、金黄色葡萄球菌和 Poly（I:C）刺激后，在转录水平上显著增加。值得注意的是，TNFR10 样在七鳃鳗外周血和神经上体的粒细胞中特异性表达。此外，在 HEK-293T 细胞中转染 tnfr10 样基因能够降低细胞活性并激活核转录因子-κB（NF-κB）。总之，这些结果表明 L-TNFR10 样可能作为七鳃鳗粒细胞中的潜在标志物发挥重要作用，并且可能自身参与免疫反应的调节。
