Vdovtseva V A, Bazhanov A N, Khlystova Z S
Arkh Anat Gistol Embriol. 1988 Jun;94(6):63-6.
Distribution of endocrine cells in composition of the secretory epithelium of the cardial glands of the human esophagus in both sex and at various age has been investigated. In spiral paraffin slices the endocrine cells have been revealed by means of different silver impregnation methods (after Grimelius, Masson--Hamperl, Sevier--Munger), Sevke technique, ferry-ferrocyanide method. Some cells have been revealed, which according to the specific signs of their granule staining resemble very much G-, EC-, ECL-cells of the stomach. They can be triangular, flatten or polygonal and are stained in the cardial gland epithelium as single diffuse cells, or as groups of cells. Staining of the slices with aldehyde-fuchsin in various modifications reveals dark cells with dark-violet granules and lighter cells with acidophilic granules. Sometimes among these cells certain cells with light-violet cytoplasm are revealed. All these cells can be arranged both in composition of the secretory epithelium of the glands and in conglomerates of cells, resembling pancreatic islands. According to their tinctorial properties they resemble A-, B-, D-cells of these islands.
对不同性别和不同年龄段的人食管贲门腺分泌上皮组成中的内分泌细胞分布进行了研究。在螺旋石蜡切片中,通过不同的银浸染方法(格里梅利乌斯法、马松 - 汉佩尔法、塞维尔 - 芒格法)、塞夫克技术、铁氰化铁 - 亚铁氰化物法揭示内分泌细胞。发现了一些细胞,根据其颗粒染色的特定特征,它们与胃的G细胞、肠嗜铬细胞(EC细胞)、肠嗜铬样细胞(ECL细胞)非常相似。它们可以是三角形、扁平形或多边形,在贲门腺上皮中呈单个散在细胞或细胞群染色。用各种改良的醛复红对切片染色可显示出带有深紫色颗粒的深色细胞和带有嗜酸性颗粒的浅色细胞。有时在这些细胞中可发现某些细胞质呈浅紫色的细胞。所有这些细胞既可以排列在腺体分泌上皮的组成中,也可以排列在类似胰岛的细胞团中。根据它们的染色特性,它们类似于这些胰岛的A细胞、B细胞、D细胞。