Rizzotti M, Domeneghini C, Castaldo L
Basic Appl Histochem. 1979;23(3):235-50.
In order to identify the endocrine cell types in various parts of the Ruminant gut, we have applied ultrastructural, both morphological and cytochemical, techniques, in parallel to the histochemical ones, to study the rectal mucosa of the adult Ox. In these studies we show that: "EC" cells, of the intestinal type, contain predominantly pleiomorphic granules, which are very electron dense and heavily reactive to "Masson" and "Grimelius" methods; "L" cells are recognizable by their numerous granules, which are fairly homogeneous in shape and osmiophilia. They do not react with "Masson" and are weak or negative to Grimelius's reaction. These granules occur near to others that are less dense, unreactive to "Masson", and that contain an argyrophilic matrix, with an eccentric electron dense core, which does not react with silver; "F-like" cells contain granules which are variable in shape, size and osmiophilia. They are unreactive to "Masson" and weak or unreactive to Grimelius silver; "H" cells contain few, small and uniformly osmiophilic granules. These are unreactive to "Masson" and uniformly reactive to "Grimelius". Our data suggest that the morphology, frequency and distribution of the cell types we have identified in the mucosa of the bovine rectum correspond with those reported in large intestine and rectum of Monogastrics, as by other authors described.
为了鉴定反刍动物肠道各部位的内分泌细胞类型，我们应用了超微结构技术，包括形态学和细胞化学技术，并与组织化学技术并行，来研究成年牛的直肠黏膜。在这些研究中我们发现：肠道型的“EC”细胞主要含有多形性颗粒，这些颗粒电子密度很高，对“Masson”法和“Grimelius”法反应强烈；“L”细胞可通过其众多颗粒识别，这些颗粒形状相当均匀，嗜锇性较强。它们对“Masson”法无反应，对Grimelius反应呈弱阳性或阴性。这些颗粒出现在其他密度较小、对“Masson”法无反应的颗粒附近，后者含有嗜银基质，有一个偏心的电子致密核心，不与银反应；“F样”细胞含有形状、大小和嗜锇性各异的颗粒。它们对“Masson”法无反应，对Grimelius银染呈弱阳性或无反应；“H”细胞含有少量、小且均匀嗜锇的颗粒。这些颗粒对“Masson”法无反应，对“Grimelius”法呈均匀反应。我们的数据表明，我们在牛直肠黏膜中鉴定出的细胞类型的形态、频率和分布与其他作者描述的单胃动物大肠和直肠中的情况相符。
