Laboratory of Applied Biochemistry and Microbiology, Department of Biochemistry, Faculty of Sciences, University of Annaba, Annaba, Algeria.
Laboratory of Applied Biochemistry and Microbiology, Department of Biochemistry, Faculty of Sciences, University of Annaba, Annaba, Algeria.
Can J Diabetes. 2018 Aug;42(4):356-364. doi: 10.1016/j.jcjd.2017.08.239. Epub 2017 Oct 14.
In diabetes, oxidative stress and lipid abnormalities are common and pronounced and represent important factors that are involved in the development of complications of diabetes. Zinc deficiency generally induces oxidative stress, but it is well known that the antioxidant Ruta chalepensis has an effective modulator role in oxidative stress in metabolic diseases. The aim of this study was to investigate the effect of R. chalepensis extract on blood biochemical parameters, tissue zinc status and antioxidant systems in rats with diabetes that were fed zinc-deficient diets.
We divided 28 male albino Wistar rats into 4 groups: 2 groups (1 group with diabetes, 1 group without diabetes) were fed zinc-sufficient diets, while the other 2 groups of rats with diabetes were fed zinc-deficient diets. One group was not treated, and the other was treated with the extract of R. chalepensis. After 3 weeks of dietary manipulation, the fasting animals were killed.
The body-weight gains of the zinc-deficient animals with diabetes were lower than those of the zinc-adequate animals with diabetes. It was noticed also that inadequate dietary zinc intake increased the glucose, cholesterol, triglyceride, urea, uric acid, creatinine and lipid peroxidation levels. In addition, the zinc-deficient diet led to a decrease in zinc tissues (femur, liver, kidney), glutathione concentration and both glutathione peroxidase and glutathione-S-transferase activities. However, R. chalepensis treatment ameliorated all the previous parameters approximately to their normal levels.
It seems that R. chalepensis supplementation is a potent factor in reducing the oxidative severity of zinc deficiency in experimental diabetes through its hypoglycemic and antioxidant actions.
在糖尿病中，氧化应激和脂质异常很常见且明显，是糖尿病并发症发生发展的重要因素。锌缺乏通常会诱导氧化应激，但众所周知，抗氧剂川续断具有在代谢性疾病的氧化应激中发挥有效调节作用。本研究旨在研究川续断提取物对喂食缺锌饮食的糖尿病大鼠血液生化参数、组织锌状态和抗氧化系统的影响。
我们将 28 只雄性白化 Wistar 大鼠分为 4 组：2 组（1 组有糖尿病，1 组无糖尿病）喂食锌充足饮食，而另外 2 组糖尿病大鼠喂食缺锌饮食。一组不治疗，另一组用川续断提取物治疗。在进行 3 周饮食干预后，处死禁食动物。
缺锌糖尿病动物的体重增长低于锌充足糖尿病动物。还注意到，锌摄入不足会增加血糖、胆固醇、甘油三酯、尿素、尿酸、肌酐和脂质过氧化水平。此外，缺锌饮食会导致锌组织（股骨、肝脏、肾脏）、谷胱甘肽浓度以及谷胱甘肽过氧化物酶和谷胱甘肽-S-转移酶活性降低。然而，川续断提取物治疗使所有上述参数大约恢复到正常水平。
川续断提取物补充似乎是通过其降血糖和抗氧化作用，减少实验性糖尿病中锌缺乏的氧化严重程度的有效因素。
