Yıldırım Metin, Erşatır Mehmet, Değirmenci Ulas, Yigin Akın, Unal Mustafa Boyraz, Guldur Muhammet Emin, Demirkol Onur, Giray Elife Sultan
Department of Pharmacy Services, Vocational School of Health Services, Tarsus University, Mersin, Turkey.
Department of Biopharmaceutics and Pharmaceutical Technology, Pharmacy Faculty, Saarland University, Saarbrücken, Germany.
Int J Environ Health Res. 2023 Dec;33(12):1533-1545. doi: 10.1080/09603123.2022.2105824. Epub 2022 Aug 2.
In this study, the protective effects of . extracts on the extent of tissue damage in gentamicin-induced nephrotoxicity have been investigated. . extracts were prepared by subcritical water and ultrasound-assisted organic solvent extraction methods. Protective activity of . extracts on Gentamicin-induced nephrotoxicity is investigated by apoptotic, DNA damage, oxidative stress markers and evaluating histopathological in kidney tissue of mice. Gentamicin significantly increased Caspase-3 and -8 activities, NO levels, serum creatinine and BUN, while 8-OHdG and MDA levels were significantly decreased with extract treatment. In addition, . extracts treatment significantly increased CAT and SOD activities. Histopathological alterations in Gentamicin group were significantly diminished by application of . extracts. These results suggest that treatment with . extracts may ameliorate renal dysfunction and structural damage through the reduction of oxidative stress and apoptosis in the kidney.
在本研究中，已对……提取物对庆大霉素诱导的肾毒性中组织损伤程度的保护作用进行了研究。……提取物通过亚临界水和超声辅助有机溶剂萃取法制备。通过凋亡、DNA损伤、氧化应激标志物以及评估小鼠肾组织的组织病理学，研究了……提取物对庆大霉素诱导的肾毒性的保护活性。庆大霉素显著增加了半胱天冬酶-3和-8的活性、一氧化氮水平、血清肌酐和血尿素氮，而……提取物处理后8-羟基脱氧鸟苷和丙二醛水平显著降低。此外，……提取物处理显著增加了过氧化氢酶和超氧化物歧化酶的活性。应用……提取物后，庆大霉素组的组织病理学改变显著减轻。这些结果表明，用……提取物治疗可能通过减少肾脏中的氧化应激和细胞凋亡来改善肾功能障碍和结构损伤。
