Department of Marine-Bio Convergence Science, Pukyong National University, Busan 48547, Republic of Korea.
Division of Food and Nutrition, Chonnam National University, Gwangju 61186, Republic of Korea.
Food Chem. 2018 Mar 1;242:156-161. doi: 10.1016/j.foodchem.2017.09.043. Epub 2017 Sep 12.
Seafood provides a range of health benefits due to its high-protein level. In this study, the osteogenic effect of blue mussel (Mytilus edulis) protein hydrolysates (BMPH) on osteoblast differentiation were examined using mouse mesenchymal stem cells (MSCs). A preparation we called BMPH<1kDa which showed the highest osteogenic effect in MSCs, was prepared by peptic hydrolysis. BMPH<1kDa treatment stimulated osteoblast differentiation with alkaline phosphatase (ALP) induction, osteocalcin and type I collagen activity as well as calcium deposition. Osteoblast differentiation stimulated by BMPH<1kDa treatment was achieved by expression of osteogenic lineage markers, such as bone morphogenetic protein-2 (BMP-2), and downstream signal and transcription factors, including p-Smad1/5/8, Dlx5, runt-related transcription factor 2 (Runx2), and osterix. BMPH<1kDa activated phosphorylation of mitogen-activated protein kinases. Adding noggin, a BMP antagonist, inhibited BMPH<1 kDa-induced ALP activity in MSCs. Taken together, our results show that BMPH<1kDa promoted osteoblast differentiation by activating BMP-2.
由于高蛋白水平，海鲜提供了一系列的健康益处。在这项研究中，使用小鼠间充质干细胞（MSCs）检查了贻贝（Mytilus edulis）蛋白水解物（BMPH）的成骨作用。通过胃蛋白酶水解制备了一种称为 BMPH<1kDa 的物质，它在 MSCs 中显示出最高的成骨作用。BMPH<1kDa 处理刺激碱性磷酸酶（ALP）诱导、骨钙素和 I 型胶原活性以及钙沉积的成骨细胞分化。BMPH<1kDa 处理刺激成骨细胞分化是通过表达成骨谱系标志物实现的，如骨形态发生蛋白-2（BMP-2），以及下游信号和转录因子，包括 p-Smad1/5/8、Dlx5、runt 相关转录因子 2（Runx2）和骨桥蛋白。BMPH<1kDa 激活丝裂原活化蛋白激酶的磷酸化。添加 BMP 拮抗剂 noggin 抑制了 MSCs 中 BMPH<1kDa 诱导的 ALP 活性。总之，我们的结果表明，BMPH<1kDa 通过激活 BMP-2 促进成骨细胞分化。
