• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

贻贝源肽 PIISVYWK 和 FSVVPSPK 触发人骨髓间充质干细胞 Wnt/β-连环蛋白信号通路介导的成骨作用。

Blue Mussel-Derived Peptides PIISVYWK and FSVVPSPK Trigger Wnt/β-Catenin Signaling-Mediated Osteogenesis in Human Bone Marrow Mesenchymal Stem Cells.

机构信息

Institute of Marine Life Sciences, Pukyong National University, Busan 48613, Korea.

Division of Food and Nutrition, Chonnam National University, Gwangju 61186, Korea.

出版信息

Mar Drugs. 2020 Oct 9;18(10):510. doi: 10.3390/md18100510.

DOI:10.3390/md18100510
PMID:33050263
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7599581/
Abstract

Marine-derived bioactive peptides have shown potential bone health promoting effects. Although various marine-derived bioactive peptides have potential nutraceutical or pharmaceutical properties, only a few of them are commercially available. This study presented an osteogenic mechanism of blue mussel-derived peptides PIISVYWK and FSVVPSPK as potential bone health promoting agents in human bone marrow-derived mesenchymal stem cells (hBMMSCs). Alkaline phosphatase (ALP) activity and mineralization were stimulated using PIISVYWK and FSVVPSPK as early and late markers of osteogenesis in a concentration-dependent manner. Western blot and RT-qPCR results revealed that PIISVYWK and FSVVPSPK increased osteoblast differentiation of hBMMSCs by activating canonical Wnt/β-catenin signaling-related proteins and mRNAs. Immunofluorescence images confirmed nuclear translocation of β-catenin in osteogenic differentiation. Treatment with the pharmacological inhibitor DKK-1 blocked PIISVYWK- and FSVVPSPK-induced ALP activity and mineralization, as well as mRNA expression of the canonical Wnt/β-catenin signaling pathway in hBMMSC differentiation into osteoblasts. These findings suggested that PIISVYWK and FSVVPSPK promoted the canonical Wnt/β-catenin signaling pathway in osteogenesis of hBMMSCs. Blue mussel-derived PIISVYWK and FSVVPSPK might help develop peptide-based therapeutic agents for bone-related diseases.

摘要

海洋来源的生物活性肽具有促进骨骼健康的潜力。虽然各种海洋来源的生物活性肽具有潜在的营养保健品或药物特性，但只有少数几种可商业化。本研究提出了贻贝来源的肽 PIISVYWK 和 FSVVPSPK 作为促进人类骨髓间充质干细胞（hBMMSCs）骨骼健康的潜在作用机制。PIISVYWK 和 FSVVPSPK 以浓度依赖的方式作为成骨早期和晚期的标记物，刺激碱性磷酸酶（ALP）活性和矿化。Western blot 和 RT-qPCR 结果表明，PIISVYWK 和 FSVVPSPK 通过激活经典 Wnt/β-连环蛋白信号相关蛋白和 mRNAs 增加 hBMMSCs 的成骨分化。免疫荧光图像证实了β-连环蛋白在成骨分化中的核转位。用药理学抑制剂 DKK-1 处理可阻断 PIISVYWK 和 FSVVPSPK 诱导的 ALP 活性和矿化，以及 hBMMSC 分化为成骨细胞过程中经典 Wnt/β-连环蛋白信号通路的 mRNA 表达。这些发现表明，PIISVYWK 和 FSVVPSPK 促进了 hBMMSCs 成骨过程中的经典 Wnt/β-连环蛋白信号通路。贻贝来源的 PIISVYWK 和 FSVVPSPK 可能有助于开发基于肽的治疗骨相关疾病的药物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac06/7599581/532f154140cc/marinedrugs-18-00510-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac06/7599581/50e386c0ddaf/marinedrugs-18-00510-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac06/7599581/b518af8c770e/marinedrugs-18-00510-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac06/7599581/12b2d9ff3dc4/marinedrugs-18-00510-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac06/7599581/12d19e89d477/marinedrugs-18-00510-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac06/7599581/dbff455282f3/marinedrugs-18-00510-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac06/7599581/2d14a068fc73/marinedrugs-18-00510-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac06/7599581/b534ce72dd55/marinedrugs-18-00510-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac06/7599581/532f154140cc/marinedrugs-18-00510-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac06/7599581/50e386c0ddaf/marinedrugs-18-00510-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac06/7599581/b518af8c770e/marinedrugs-18-00510-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac06/7599581/12b2d9ff3dc4/marinedrugs-18-00510-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac06/7599581/12d19e89d477/marinedrugs-18-00510-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac06/7599581/dbff455282f3/marinedrugs-18-00510-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac06/7599581/2d14a068fc73/marinedrugs-18-00510-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac06/7599581/b534ce72dd55/marinedrugs-18-00510-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac06/7599581/532f154140cc/marinedrugs-18-00510-g008.jpg

相似文献

1
Blue Mussel-Derived Peptides PIISVYWK and FSVVPSPK Trigger Wnt/β-Catenin Signaling-Mediated Osteogenesis in Human Bone Marrow Mesenchymal Stem Cells.贻贝源肽 PIISVYWK 和 FSVVPSPK 触发人骨髓间充质干细胞 Wnt/β-连环蛋白信号通路介导的成骨作用。
Mar Drugs. 2020 Oct 9;18(10):510. doi: 10.3390/md18100510.
2
Anti-Osteoporotic Effects of Antioxidant Peptides PIISVYWK and FSVVPSPK from on Ovariectomized Mice.抗氧化肽PIISVYWK和FSVVPSPK对去卵巢小鼠的抗骨质疏松作用
Antioxidants (Basel). 2020 Sep 15;9(9):866. doi: 10.3390/antiox9090866.
3
Berberine promotes bone marrow-derived mesenchymal stem cells osteogenic differentiation via canonical Wnt/β-catenin signaling pathway.黄连素通过经典Wnt/β-连环蛋白信号通路促进骨髓间充质干细胞成骨分化。
Toxicol Lett. 2016 Jan 5;240(1):68-80. doi: 10.1016/j.toxlet.2015.10.007. Epub 2015 Oct 22.
4
FHL2 mediates dexamethasone-induced mesenchymal cell differentiation into osteoblasts by activating Wnt/beta-catenin signaling-dependent Runx2 expression.FHL2通过激活Wnt/β-连环蛋白信号通路依赖的Runx2表达，介导地塞米松诱导间充质细胞分化为成骨细胞。
FASEB J. 2008 Nov;22(11):3813-22. doi: 10.1096/fj.08-106302. Epub 2008 Jul 24.
5
Polydatin promotes the osteogenic differentiation of human bone mesenchymal stem cells by activating the BMP2-Wnt/β-catenin signaling pathway.虎杖苷通过激活 BMP2-Wnt/β-catenin 信号通路促进人骨髓间充质干细胞的成骨分化。
Biomed Pharmacother. 2019 Apr;112:108746. doi: 10.1016/j.biopha.2019.108746. Epub 2019 Mar 2.
6
Cajanolactone A from Promoted Osteoblast Differentiation in Human Bone Marrow Mesenchymal Stem Cells via Stimulating Wnt/LRP5/β-Catenin Signaling.卡楠内酯 A 可通过激活 Wnt/LRP5/β-连环蛋白信号通路促进人骨髓间充质干细胞成骨分化。
Molecules. 2019 Jan 12;24(2):271. doi: 10.3390/molecules24020271.
7
Fluoride promotes osteoblastic differentiation through canonical Wnt/β-catenin signaling pathway.氟化物通过经典 Wnt/β-连环蛋白信号通路促进成骨细胞分化。
Toxicol Lett. 2014 Feb 10;225(1):34-42. doi: 10.1016/j.toxlet.2013.11.029. Epub 2013 Dec 1.
8
Ark shell protein-derived bioactive peptides promote osteoblastic differentiation through upregulation of the canonical Wnt/β-catenin signaling in human bone marrow-derived mesenchymal stem cells.蚶壳蛋白衍生的生物活性肽通过上调人骨髓间充质干细胞中经典的Wnt/β-连环蛋白信号通路来促进成骨细胞分化。
J Food Biochem. 2020 Oct;44(10):e13440. doi: 10.1111/jfbc.13440. Epub 2020 Aug 18.
9
Icariin stimulates osteogenesis and suppresses adipogenesis of human bone mesenchymal stem cells via miR-23a-mediated activation of the Wnt/β-catenin signaling pathway.淫羊藿苷通过miR-23a介导的Wnt/β-连环蛋白信号通路激活刺激人骨髓间充质干细胞的成骨作用并抑制其脂肪生成。
Phytomedicine. 2021 May;85:153485. doi: 10.1016/j.phymed.2021.153485. Epub 2021 Jan 29.
10
Flavonoids of Herba Epimedii regulate osteogenesis of human mesenchymal stem cells through BMP and Wnt/beta-catenin signaling pathway.淫羊藿黄酮通过 BMP 和 Wnt/β-catenin 信号通路调控人骨髓间充质干细胞成骨分化。
Mol Cell Endocrinol. 2010 Jan 15;314(1):70-4. doi: 10.1016/j.mce.2009.08.012. Epub 2009 Aug 22.

引用本文的文献

1
Blue Mussel-Derived Bioactive Peptides PIISVYWK (P1) and FSVVPSPK (P2): Promising Agents for Inhibiting Foam Cell Formation and Inflammation in Cardiovascular Diseases.蓝贻贝源生物活性肽 PIISVYWK(P1) 和 FSVVPSPK(P2)：抑制心血管疾病泡沫细胞形成和炎症的有潜力药物。
Mar Drugs. 2024 Oct 10;22(10):466. doi: 10.3390/md22100466.
2
Metabolic bone disorders and the promise of marine osteoactive compounds.代谢性骨病与海洋活性化合物的前景。
Cell Mol Life Sci. 2023 Dec 20;81(1):11. doi: 10.1007/s00018-023-05033-x.
3
The recent progress of peptide regulators for the Wnt/β-catenin signaling pathway.

本文引用的文献

1
Ark shell protein-derived bioactive peptides promote osteoblastic differentiation through upregulation of the canonical Wnt/β-catenin signaling in human bone marrow-derived mesenchymal stem cells.蚶壳蛋白衍生的生物活性肽通过上调人骨髓间充质干细胞中经典的Wnt/β-连环蛋白信号通路来促进成骨细胞分化。
J Food Biochem. 2020 Oct;44(10):e13440. doi: 10.1111/jfbc.13440. Epub 2020 Aug 18.
2
Two novel peptides from ark shell protein stimulate osteoblast differentiation and rescue ovariectomy-induced bone loss.两种新型来自贝壳蛋白的肽刺激成骨细胞分化并挽救去卵巢导致的骨丢失。
Toxicol Appl Pharmacol. 2019 Dec 15;385:114779. doi: 10.1016/j.taap.2019.114779. Epub 2019 Nov 4.
3
Wnt/β-连环蛋白信号通路肽调节剂的最新进展
Front Med (Lausanne). 2023 Jun 16;10:1164656. doi: 10.3389/fmed.2023.1164656. eCollection 2023.
4
Repair and Mechanism of Oligopeptide SEP-3 on Oxidative Stress Liver Injury Induced by Sleep Deprivation in Mice.寡肽 SEP-3 对睡眠剥夺诱导的小鼠氧化应激性肝损伤的修复作用及机制。
Mar Drugs. 2023 Feb 22;21(3):139. doi: 10.3390/md21030139.
5
Cytoprotective Peptides from Blue Mussel Protein Hydrolysates: Identification and Mechanism Investigation in Human Umbilical Vein Endothelial Cells Injury.蓝贻贝蛋白水解物的细胞保护肽：人脐静脉内皮细胞损伤中的鉴定和机制研究。
Mar Drugs. 2021 Oct 27;19(11):609. doi: 10.3390/md19110609.
6
Biological Functions of Let-7e-5p in Promoting the Differentiation of MC3T3-E1 Cells.Let-7e-5p在促进MC3T3-E1细胞分化中的生物学功能
Front Cell Dev Biol. 2021 Sep 8;9:671170. doi: 10.3389/fcell.2021.671170. eCollection 2021.
7
BK Channel Deficiency in Osteoblasts Reduces Bone Formation via the Wnt/β-Catenin Pathway.成骨细胞中 BK 通道缺失通过 Wnt/β-连环蛋白通路减少骨形成。
Mol Cells. 2021 Aug 31;44(8):557-568. doi: 10.14348/molcells.2021.0004.
8
Plasma Spray vs. Electrochemical Deposition: Toward a Better Osteogenic Effect of Hydroxyapatite Coatings on 3D-Printed Titanium Scaffolds.等离子喷涂与电化学沉积：实现羟基磷灰石涂层在3D打印钛支架上更好的成骨效果
Front Bioeng Biotechnol. 2021 Jul 26;9:705774. doi: 10.3389/fbioe.2021.705774. eCollection 2021.
Bone health-promoting bioactive peptides.
促进骨骼健康的生物活性肽。
J Food Biochem. 2019 Jan;43(1):e12529. doi: 10.1111/jfbc.12529. Epub 2018 Feb 21.
4
Collagen Peptide Upregulates Osteoblastogenesis from Bone Marrow Mesenchymal Stem Cells through MAPK- Runx2.胶原肽通过 MAPK-Runx2 上调骨髓间充质干细胞成骨分化。
Cells. 2019 May 11;8(5):446. doi: 10.3390/cells8050446.
5
Amino Acid Composition, Antioxidant, and Cytoprotective Effect of Blue Mussel () Hydrolysate through the Inhibition of Caspase-3 Activation in Oxidative Stress-Mediated Endothelial Cell Injury.蓝贻贝（）水解物的氨基酸组成、抗氧化和细胞保护作用通过抑制氧化应激介导的内皮细胞损伤中的半胱天冬酶-3 活化。
Mar Drugs. 2019 Feb 25;17(2):135. doi: 10.3390/md17020135.
6
Sea Squirt (Halocynthia roretzi) Hydrolysates Induce Apoptosis in Human Colon Cancer HT-29 Cells through Activation of Reactive Oxygen Species.海鞘（Halocynthia roretzi）水解物通过激活活性氧诱导人结肠癌细胞 HT-29 细胞凋亡。
Nutr Cancer. 2019;71(1):118-127. doi: 10.1080/01635581.2018.1540717. Epub 2019 Feb 9.
7
Protective effect of enzymatic hydrolysates from seahorse (Hippocampus abdominalis) against HO-mediated human umbilical vein endothelial cell injury.海马（腹海马）酶解产物对过氧化氢介导的人脐静脉内皮细胞损伤的保护作用。
Biomed Pharmacother. 2018 Dec;108:103-110. doi: 10.1016/j.biopha.2018.08.143. Epub 2018 Sep 12.
8
Regulation of Osteoblast Metabolism by Wnt Signaling.Wnt信号通路对成骨细胞代谢的调控
Endocrinol Metab (Seoul). 2018 Sep;33(3):318-330. doi: 10.3803/EnM.2018.33.3.318. Epub 2018 Aug 14.
9
Simvastatin induces osteogenic differentiation of MSCs via Wnt/β-catenin pathway to promote fracture healing.辛伐他汀通过 Wnt/β-连环蛋白通路诱导间充质干细胞成骨分化，促进骨折愈合。
Eur Rev Med Pharmacol Sci. 2018 May;22(9):2896-2905. doi: 10.26355/eurrev_201805_14992.
10
Blue mussel (Mytilus edulis) protein hydrolysate promotes mouse mesenchymal stem cell differentiation into osteoblasts through up-regulation of bone morphogenetic protein.贻贝（Mytilus edulis）蛋白水解物通过上调骨形态发生蛋白促进小鼠间充质干细胞向成骨细胞分化。
Food Chem. 2018 Mar 1;242:156-161. doi: 10.1016/j.foodchem.2017.09.043. Epub 2017 Sep 12.