D'Onofrio Claudio, Matarese Fabiola, Cuzzola Angela
Department of Agriculture, Food and Environment, University of Pisa, Via del Borghetto 80, I-56124 Pisa, Italy; Nutraceuticals and Food for Health - Nutrafood, University of Pisa, Via del Borghetto 80, I-56124 Pisa, Italy.
Department of Agriculture, Food and Environment, University of Pisa, Via del Borghetto 80, I-56124 Pisa, Italy.
Food Chem. 2018 Mar 1;242:352-361. doi: 10.1016/j.foodchem.2017.09.084. Epub 2017 Sep 18.
Methyl jasmonate (MeJA) was applied in a vineyard on leaves and grape clusters of cv Sangiovese to test its ability to stimulate the production of aromas and identify the main genes involved in the biosynthetic pathways switched on by the elicitor. MeJA application led to a delay in grape technological maturity and a significant increase in the concentration of several berry aroma classes (about twice the total aroma: from around 3 to 6μg/g of berry). Of these, monoterpenes showed the most significant increase. An analysis of the expression of terpenoid biosynthesis genes confirmed that the MeJA application activated the related biosynthetic pathway. The expression of all the TPS genes analyzedwas higher in samples treated with MeJA. Also the wines produced by microvinification of Sangiovese treated and untreated grapes showed a rise in the aroma concentration as in berries, with an important impact on longevity and sensorial characters of wines.
在一个葡萄园里,将茉莉酸甲酯(MeJA)施用于桑娇维塞(Sangiovese)葡萄品种的叶片和葡萄串上,以测试其刺激香气产生的能力,并确定参与由该诱导剂开启的生物合成途径的主要基因。施用MeJA导致葡萄工艺成熟延迟,几种浆果香气类别的浓度显著增加(总香气约增加两倍:从浆果中约3μg/g增加到6μg/g左右)。其中,单萜类化合物的增加最为显著。对萜类生物合成基因表达的分析证实,施用MeJA激活了相关的生物合成途径。在MeJA处理的样品中,所有分析的TPS基因的表达都更高。用处理过和未处理过的桑娇维塞葡萄进行微型酿酒所生产的葡萄酒,其香气浓度也如浆果中一样有所上升,对葡萄酒的陈酿能力和感官特征有重要影响。