苦瓜中基因的鉴定与表达分析()。 （注：括号部分原文缺失具体内容）
Identification and Expression Analysis of Gene in Bitter Gourd ().
作者信息
Ge Haicui, Liu Shuang, Zheng Hongzhe, Chang Pengyan, Huang Weiqun, Lin Shanshan, Zheng Jingyuan, Li Honglong, Huang Zedong, Jia Qi, Zhong Fenglin
机构信息
College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Fuzhou Smart Agriculture (Seed Industry) Industry Innovation Center, Fuzhou 350002, China.
出版信息
Genes (Basel). 2024 Nov 29;15(12):1557. doi: 10.3390/genes15121557.
BACKGROUND
Lipoxygenases (LOXs) are key enzymes in the unsaturated fatty acid oxidation reaction pathway and play an important regulatory role in the synthesis of fruit aroma volatiles.
METHODS
gene family members were identified in the whole genome database of bitter gourd and analyzed bioinformatically. An RT-qPCR was used to analyze the expression differences in different tissues. Monoterpenes were determined by gas chromatography-mass spectrometry (GC-MS) technique.
RESULTS
A total of 12 gene family members were identified in the genome. The expression of genes varied significantly among the tissues of roots, stems, leaves, flowers, fruits, seeds and tendrils. A total of 29 monoterpenes were detected in the fruits of five different fruit colors of bitter gourd, mainly containing six types of alcohols, aldehydes, terpenes, ketones, esters and alkynes, with the highest relative content of alcohols.
CONCLUSIONS
The present study provides a reference for further elucidation of the biological functions of the gene in the synthesis pathway of aroma volatiles in bitter gourd.
背景
脂氧合酶（LOXs）是不饱和脂肪酸氧化反应途径中的关键酶，在果实香气挥发物的合成中起重要调节作用。
方法
在苦瓜全基因组数据库中鉴定基因家族成员并进行生物信息学分析。采用逆转录定量聚合酶链反应（RT-qPCR）分析不同组织中的表达差异。通过气相色谱-质谱联用（GC-MS）技术测定单萜类化合物。
结果
在基因组中总共鉴定出12个基因家族成员。这些基因在根、茎、叶、花、果实、种子和卷须的组织中表达差异显著。在五种不同果实颜色的苦瓜果实中总共检测到29种单萜类化合物，主要含有醇、醛、萜烯、酮、酯和炔六种类型，其中醇的相对含量最高。
结论
本研究为进一步阐明该基因在苦瓜香气挥发物合成途径中的生物学功能提供了参考。
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