Division of Biotechnology, Department of Chemistry, Lund University, P.O. Box 124, SE-22100 Lund, Sweden.
Food Chem. 2018 Mar 1;242:579-584. doi: 10.1016/j.foodchem.2017.09.048. Epub 2017 Sep 12.
An arabinose specific xylanase from glycoside hydrolase family 5 (GH5) was used to hydrolyse wheat and rye arabinoxylan, and the product profile showed that it produced arabinose substituted oligosaccharides (AXOS) having 2-10 xylose residues in the main chain but no unsubstituted xylooligosaccharides (XOS). Molecular modelling showed that the active site has an open structure and that the hydroxyl groups of all xylose residues in the active site are solvent exposed, indicating that arabinose substituents can be accommodated in the glycone as well as the aglycone subsites. The arabinoxylan hydrolysates obtained with the GH5 enzyme stimulated growth of Bifidobacterium adolescentis but not of Lactobacillus brevis. This arabinoxylanase is thus a good tool for the production of selective prebiotics.
一种来源于糖苷水解酶家族 5（GH5）的阿拉伯糖特异性木聚糖酶被用于水解小麦和黑麦阿拉伯木聚糖，其产物谱表明它产生了具有 2-10 个木糖残基主链的阿拉伯糖取代低聚糖（AXOS），但没有未取代的木低聚糖（XOS）。分子建模表明，活性位点具有开放结构，并且活性位点中所有木糖残基的羟基都暴露于溶剂中，表明阿拉伯糖取代基可以容纳在糖基和非糖基部位。用 GH5 酶获得的阿拉伯木聚糖水解产物刺激了青春双歧杆菌的生长，但不刺激短乳杆菌的生长。因此，这种阿拉伯木聚糖酶是生产选择性益生元的良好工具。
