Faculty of Agro-Industry, Chiang Mai University, Chiang Mai, 50100, Thailand.
Cluster of High Value Products from Thai Rice and Plants for Health, Chiang Mai University, Chiang Mai, 50100, Thailand.
Sci Rep. 2022 Jan 7;12(1):11. doi: 10.1038/s41598-021-03360-2.
The prebiotic properties of xylooligosaccharides (XOS) and arabino-xylooligosaccharides (AXOS) produced from rice husk (RH) using microwave treatment combined with enzymatic hydrolysis were evaluated. The RH was subjected to microwave pretreatment at 140, 160 and 180 °C for 5, 10 and 15 min to obtain crude arabinoxylan (AX). Increasing microwave pretreatment time increased sugar content. Crude AX was extracted with 2% (w/v) sodium hydroxide at 25 °C for 24 h and used as a substrate for XOS production by commercial xylanases. Results showed that oligosaccharides produced by Pentopan Mono BG and Ultraflo Max provided xylobiose and xylotriose as the main products. AXOS was also present in the oligosaccharides that promoted growth of Lactobacillus spp. and resisted degradation by over 70% after exposure to simulated human digestion.
采用微波处理结合酶解从米糠中制备的木二糖（XOS）和阿拉伯木二糖（AXOS）的益生元特性进行了评价。米糠经 140、160 和 180°C 微波预处理 5、10 和 15 min 以获得粗阿拉伯木聚糖（AX）。微波预处理时间的增加提高了糖含量。粗 AX 用 2%（w/v）氢氧化钠在 25°C 下提取 24 小时，并用作商业木聚糖酶生产 XOS 的底物。结果表明，Pentopan Mono BG 和 Ultraflo Max 产生的低聚糖以木二糖和木三糖为主要产物。AXOS 也存在于低聚糖中，促进了乳酸菌的生长，并在模拟人体消化后仍有超过 70%的抗性。
