Gao Lingfeng, Li Xiang, Li Haiying, Li Xiang, Li Jinquan, Shen Haitao, Chen Gang
Department of Neurosurgery & Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, China.
Department of Neurosurgery & Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, China.
Neurosci Lett. 2018 Jan 1;662:356-360. doi: 10.1016/j.neulet.2017.10.021. Epub 2017 Oct 14.
N-myc downstream regulated gene 2 (NDRG2) was a member of the N-myc down regulated gene family which belongs to the alpha/beta hydrolase superfamily and played important roles in cell death. To date, the expression and effects of NDRG2 in brain after intracerebral hemorrhage (ICH) are unclear. In this study, we investigated the spatial-temporal expression of NDRG2 in brain tissues in a rat model of ICH. The expression levels of NDRG2 were tested in 3h, 6h, 12h, 24h, 48h, 72h, and 7d after ICH by western blot analysis. The results showed that the NDRG2 levels were increased and peaked at 24h after ICH, and then declined subsequently. Meanwhile, we also examined the NDRG2 cellular localization in brain tissues by immunofluorescence analysis with NeuN and GFAP (biomarker of neuron and astrocytes respectively). The results demonstrated that NDRG2 was mainly expressed in astrocytes, but not neurons, after ICH. Additionally, the results of double staining indicated that the rate of NDRG2- and TUNEL -positive cells was significantly higher in the brain tissues in rats after ICH. The roles of NDRG2 in ICH needed further investigation and inhibiting the expression of NDRG2 may have potential therapeutic effects in ICH.
N-myc下游调控基因2（NDRG2）是N-myc下调基因家族的成员，属于α/β水解酶超家族，在细胞死亡中发挥重要作用。迄今为止，脑出血（ICH）后NDRG2在脑中的表达及作用尚不清楚。在本研究中，我们在大鼠ICH模型中研究了NDRG2在脑组织中的时空表达。通过蛋白质印迹分析检测ICH后3小时、6小时、12小时、24小时、48小时、72小时和7天的NDRG2表达水平。结果显示，ICH后NDRG2水平升高，并在24小时达到峰值，随后下降。同时，我们还通过分别用NeuN和GFAP（神经元和星形胶质细胞的生物标志物）进行免疫荧光分析来检测脑组织中NDRG2的细胞定位。结果表明，ICH后NDRG2主要在星形胶质细胞中表达，而不在神经元中表达。此外，双重染色结果表明，ICH后大鼠脑组织中NDRG2和TUNEL阳性细胞的比例显著更高。NDRG2在ICH中的作用需要进一步研究，抑制NDRG2的表达可能对ICH具有潜在的治疗作用。
