Reininger L, Kaushik A, Izui S, Jaton J C
Department of Medical Biochemistry, Medical Center of the University of Geneva, Switzerland.
Eur J Immunol. 1988 Oct;18(10):1521-6. doi: 10.1002/eji.1830181008.
A cDNA clone encoding the variable region of the heavy chain of a BALB/c antibromelinized mouse red blood cell (BrMRBC) monoclonal antibody has been characterized. The nucleic acid sequence indicates that the variable region of the heavy chain is likely encoded by variable-VCP12, diversity-DSP 2 (5, 7 or 8) and joining-JH1 germ-line genes. An identical combination of V genes was observed for six other CBA/J and NZB anti-BrMRBC hybridomas. The BALB/c VCP12 nucleotide sequence is less than 80% homologous to members of the 10 known VH families. Moreover, the genomic restriction fragments detected under moderate stringency conditions with the radiolabeled cDNA probe did not correspond to those obtained with probes of the most homologous VH families (7183 and X24). The results indicate that the VCP12 gene defines a new VH family which we propose to designate VH11. The observation of 1, 2 or 3 genomic restriction fragments at the most, with mice bearing the Igh-Vd or j, Igh-Va or b or Igh-Vc haplotypes, suggests the existence of a few VCP12-related genes.
一个编码BALB/c抗菠萝蛋白酶处理的小鼠红细胞（BrMRBC）单克隆抗体重链可变区的cDNA克隆已被鉴定。核酸序列表明，重链可变区可能由可变区VCP12、多样性区DSP 2（5、7或8）和连接区JH1种系基因编码。在其他六个CBA/J和NZB抗BrMRBC杂交瘤中观察到了相同的V基因组合。BALB/c VCP12核苷酸序列与10个已知VH家族成员的同源性低于80%。此外，用放射性标记的cDNA探针在中等严格条件下检测到的基因组限制性片段与用最同源的VH家族（7183和X24）的探针获得的片段不对应。结果表明，VCP12基因定义了一个新的VH家族，我们建议将其命名为VH11。对于携带Igh-Vd或j、Igh-Va或b或Igh-Vc单倍型的小鼠，最多观察到1、2或3个基因组限制性片段，这表明存在一些与VCP12相关的基因。
