Bazhan Nadezhda M, Baklanov Alexandr V, Piskunova Julia V, Kazantseva Antonina J, Makarova Elena N
Laboratory of Physiological Genetics, The Siberian Branch of the Russian Academy of Sciences, The Federal Research Center Institute of Cytology and Genetics, Novosibirsk, Russia
Laboratory of Physiological Genetics, The Siberian Branch of the Russian Academy of Sciences, The Federal Research Center Institute of Cytology and Genetics, Novosibirsk, Russia.
Physiol Rep. 2017 Oct;5(19). doi: 10.14814/phy2.13445. Epub 2017 Oct 16.
C57Bl mice exhibit impaired glucose metabolism by the late adult age under standard living conditions. The aim of this study was to evaluate white adipose tissue (WAT), brown adipose tissue (BAT), and skeletal muscle expression of genes involved in carbohydrate-lipid metabolism at postpubertal stages preceding the late adult age in C57Bl mice. Muscle mRNA levels of uncoupling protein 3 () and carnitine palmitoyltransferase 1 () (indicators of FFA oxidation), WAT mRNA levels of hormone-sensitive lipase () and lipoprotein lipase () (indicators of lipolysis and lipogenesis), muscle and WAT mRNA levels of the type 4 glucose transporter (indicators of insulin-dependent glucose uptake), and BAT mRNA levels of uncoupling protein 1 () (indicator of thermogenesis) were measured in fed and 16 h-fasted mice in three age groups: 10-week-old (young), 15-week-old (early adult), and 30-week-old (late adult). Weight gain from young to early adult age was not accompanied by changes in WAT and BAT indexes and biochemical blood parameters. Weight gain from early to late adult age was accompanied by increased WAT and BAT indexes and decreased glucose tolerance. Muscle and mRNA levels and WAT and mRNA levels increased from young to early adult age and then sharply decreased by the late adult age. Moreover, BAT mRNA level decreased in the late adult age. Fasting failed to increase muscle Cpt1 mRNA levels in late adult mice. These transcriptional changes could contribute to impaired glucose metabolism and the onset of obesity in late adult mice during normal development.
在标准生活条件下，C57Bl小鼠成年后期会出现葡萄糖代谢受损。本研究的目的是评估C57Bl小鼠成年后期之前青春期后阶段白色脂肪组织（WAT）、棕色脂肪组织（BAT）和骨骼肌中参与碳水化合物-脂质代谢的基因表达。在三个年龄组（10周龄（年轻）、15周龄（成年早期）和30周龄（成年后期））的喂食小鼠和禁食16小时的小鼠中，测量了解偶联蛋白3（）和肉碱棕榈酰转移酶1（）（游离脂肪酸氧化指标）的肌肉mRNA水平、激素敏感性脂肪酶（）和脂蛋白脂肪酶（）（脂解和脂肪生成指标）的WAT mRNA水平、4型葡萄糖转运蛋白（胰岛素依赖性葡萄糖摄取指标）的肌肉和WAT mRNA水平以及解偶联蛋白1（产热指标）的BAT mRNA水平。从幼年到成年早期体重增加并未伴随WAT和BAT指数以及血液生化参数的变化。从成年早期到成年后期体重增加伴随着WAT和BAT指数增加以及葡萄糖耐量降低。肌肉和mRNA水平以及WAT和mRNA水平从幼年到成年早期增加，然后在成年后期急剧下降。此外，成年后期BAT mRNA水平降低。禁食未能增加成年后期小鼠肌肉Cpt1 mRNA水平。这些转录变化可能导致成年后期小鼠在正常发育过程中葡萄糖代谢受损和肥胖的发生。
