National Engineering Laboratory for Animal Breeding and Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, P.R. China.
Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, 810001, P.R. China.
Sci Rep. 2017 Oct 16;7(1):13245. doi: 10.1038/s41598-017-13381-5.
In vitro maturation (IVM) and vitrification have been widely used to prepare oocytes before fertilization; however, potential effects of these procedures, such as expression profile changes, are poorly understood. In this study, mouse oocytes were divided into four groups and subjected to combinations of in vitro maturation and/or vitrification treatments. RNA-seq and in silico pathway analysis were used to identify differentially expressed genes (DEGs) that may be involved in oocyte viability after in vitro maturation and/or vitrification. Our results showed that 1) 69 genes were differentially expressed after IVM, 66 of which were up-regulated. Atp5e and Atp5o were enriched in the most significant gene ontology term "mitochondrial membrane part"; thus, these genes may be promising candidate biomarkers for oocyte viability after IVM. 2) The influence of vitrification on the transcriptome of oocytes was negligible, as no DEGs were found between vitrified and fresh oocytes. 3) The MII stage is more suitable for oocyte vitrification with respect to the transcriptome. This study provides a valuable new theoretical basis to further improve the efficiency of in vitro maturation and/or oocyte vitrification.
体外成熟（IVM）和玻璃化已广泛用于受精前卵母细胞的制备；然而，这些程序的潜在影响，如表达谱的变化，还知之甚少。在这项研究中，将小鼠卵母细胞分为四组，并进行体外成熟和/或玻璃化处理的组合。使用 RNA-seq 和计算机途径分析来鉴定可能参与体外成熟和/或玻璃化后卵母细胞活力的差异表达基因（DEGs）。我们的结果表明：1）IVM 后有 69 个基因表达差异，其中 66 个基因上调。Atp5e 和 Atp5o 在最显著的基因本体术语“线粒体膜部分”中富集，因此这些基因可能是 IVM 后卵母细胞活力的有前途的候选生物标志物。2）玻璃化对卵母细胞转录组的影响可以忽略不计，因为玻璃化和新鲜卵母细胞之间没有发现差异表达基因。3）MII 期在转录组方面更适合卵母细胞玻璃化。这项研究为进一步提高体外成熟和/或卵母细胞玻璃化的效率提供了有价值的新理论基础。
