Liu Lifeng
Energy Bioscience Institute, University of California, 2151 Berkeley Way, Berkeley, CA, 94720, USA.
, 2800 Mitchell Drive, B400-427, Walnut Creek, CA, 94598, USA.
Methods Mol Biol. 2018;1667:65-71. doi: 10.1007/978-1-4939-7278-4_6.
Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) has become an important tool for the analysis of biomolecules, such as DNA, peptides, and oligosaccharides. This technique has been developed as a rapid, sensitive, and accurate means for analyzing cell wall polysaccharide structures. Here, we describe a method using mass spectrometry to provide xyloglucan composition and structure information of Brachypodium plants which will be useful for functional characterization of xyloglucan biosynthesis pathway in Brachypodium distachyon.
基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）已成为分析生物分子（如DNA、肽和寡糖）的重要工具。该技术已发展成为一种用于分析细胞壁多糖结构的快速、灵敏且准确的方法。在此，我们描述了一种使用质谱法来提供短柄草属植物木葡聚糖组成和结构信息的方法，这将有助于对二穗短柄草中木葡聚糖生物合成途径进行功能表征。
