Kruse Thomas, Goris Tobias, Maillard Julien, Woyke Tanja, Lechner Ute, de Vos Willem, Smidt Hauke
Laboratory of Microbiology, Wageningen University, Stippeneng 4, 6708 WE Wageningen, The Netherlands.
Department of Applied and Ecological Microbiology, Friedrich-Schiller-University Jena, Philosophenweg 12, 07743 Jena, Germany.
FEMS Microbiol Ecol. 2017 Dec 1;93(12). doi: 10.1093/femsec/fix135.
The Desulfitobacterium genus comprises anaerobic Gram-positive bacteria, of which the majority are facultative organohalide respirers. We here present the genomes of eight strains of Desulfitobacterium spp., including five strains of Desulfitobacterium hafniense, one strain each from D. dichloroeliminans and D. metallireducens, and one strain that had not been assigned to any species prior to this study. The newly sequenced genomes were compared with four previously published desulfitobacterial genomes. The average genome sizes are 5.5, 4.3 and 3.4 Mbp for D. hafniense, D. dehalogenans and D. dichloroeliminans/metallireducens, respectively. The genomes encode up to seven reductive dehalogenases, the genomes of both D. hafniense DP7 and D. metallireducens 853-15AT did not encode any reductive dehalogenase. The latter result was a surprise as D. metallireducens 853-15AT has been reported to carry out organohalide respiration. Unlike reported for the pceABCT gene cluster, the other reductive dehalogenase gene clusters do not show any signs of being genetically mobile. All analyzed desulfitobacterial genomes encode a complete cobalamin synthesis pathway. A menaquinone synthesis pathway was found in all strains except D. dichloroeliminans DCA1T. The detailed analysis of the genome sequence of 12 desulfitobacteria from four different species confirmed that this genus has an extremely large metabolic repertoire.
脱硫肠状菌属包含厌氧革兰氏阳性菌，其中大多数是兼性有机卤化物呼吸菌。我们在此展示了8株脱硫肠状菌属菌株的基因组，包括5株哈氏脱硫肠状菌、1株二氯消除脱硫肠状菌和1株金属还原脱硫肠状菌，以及1株在本研究之前未被归入任何物种的菌株。将新测序的基因组与之前公布的4个脱硫肠状菌基因组进行了比较。哈氏脱硫肠状菌、脱卤脱硫肠状菌和二氯消除脱硫肠状菌/金属还原脱硫肠状菌的平均基因组大小分别为5.5、4.3和3.4 Mbp。这些基因组编码多达7种还原性脱卤酶，哈氏脱硫肠状菌DP7和金属还原脱硫肠状菌853-15AT的基因组均未编码任何还原性脱卤酶。后一结果令人惊讶，因为据报道金属还原脱硫肠状菌853-15AT能进行有机卤化物呼吸。与pceABCT基因簇的报道不同，其他还原性脱卤酶基因簇没有显示出任何基因移动的迹象。所有分析的脱硫肠状菌基因组都编码一条完整的钴胺素合成途径。除二氯消除脱硫肠状菌DCA1T外，所有菌株中都发现了甲萘醌合成途径。对来自4个不同物种的12株脱硫肠状菌的基因组序列进行的详细分析证实，该属具有极其丰富的代谢能力。
