Bong Lee-Jin, Tu Wu-Chun, Neoh Kok-Boon, Huang Chin-Gi, Ting Rou-Xing
National Mosquito-borne Diseases Control Research Center, National Health Research Institute, Zhunan, Miaoli County, Taiwan, ROC.
Department of Entomology, National Chung Hsing University, Taichung, Taiwan, ROC.
J Med Entomol. 2018 Jan 10;55(1):36-42. doi: 10.1093/jme/tjx191.
The phenomenon of high egg reproduction when mortality risk rises is common in mosquitoes. However, the phenomenon may vary between insecticide susceptible and field-collected strains, due to the latter's decreased energy allocation in reproduction in the presence of insecticide resistance. In this study, we evaluated the effect of chlorpyrifos (CP) and temephos (TP) exposure on the oviposition and survival of Aedes aegypti (Linnaeus) (Diptera: Culicidae) using a susceptible strain (KHsm) and two field strains (KHly and TNnorth). We also dissected the female mosquitoes of each strain on fifth day after the first blood meal to examine the total number of eggs produced. Neither CP nor TP exhibited oviposition deterrent against female mosquitoes of any of the three strains, as the females did not show decreased reproduction activity on the insecticide-treated sites. Of the two insecticides tested, only CP had an adulticidal effect on Ae. aegypti. High mortality was recorded in KHsm after contacting the CP-treated oviposition sites on day 4. Before death, KHsm mosquitoes oviposited significantly more eggs compared to the two field strains. However, the difference of total egg production between susceptible and field-collected strains was subtle. Thus, the decreased reproductive output in field-collected strains might not be directly linked to energy and resource allocation. In this respect, we should consider the possible involvement of biogenic amines in the egg retention in field-collected strains when mortality risk rises. The phenomenon was not observed in nonadulticidal TP treatment.
在蚊子中，死亡率风险上升时卵繁殖率高的现象很常见。然而，由于野外采集的品系在存在抗药性时繁殖中的能量分配减少，这种现象在对杀虫剂敏感的品系和野外采集的品系之间可能有所不同。在本研究中，我们使用一个敏感品系（KHsm）和两个野外品系（KHly和TNnorth）评估了毒死蜱（CP）和双硫磷（TP）暴露对埃及伊蚊（Linnaeus）（双翅目：蚊科）产卵和存活的影响。我们还在首次吸血后第5天解剖了每个品系的雌蚊，以检查所产的卵总数。CP和TP对这三个品系的雌蚊均未表现出产卵抑制作用，因为雌蚊在经杀虫剂处理的场所并未表现出繁殖活动减少。在所测试的两种杀虫剂中，只有CP对埃及伊蚊具有成虫杀灭作用。在第4天接触经CP处理的产卵场所后，KHsm品系记录到高死亡率。在死亡前，KHsm品系的蚊子产卵量明显多于两个野外品系。然而，敏感品系和野外采集品系之间的总产卵量差异细微。因此，野外采集品系繁殖产出的减少可能与能量和资源分配没有直接关联。在这方面，当死亡率风险上升时，我们应考虑生物胺可能参与了野外采集品系的卵滞留。在非成虫杀灭性的TP处理中未观察到这种现象。
