Holzmayer T A, Karataev G I, Rozinov M N, Moskvina I L, Shumakov Y L, Motin V L, Mebel S M, Gershanovich V N, Lapaeva I A
Gamaleya Institute of Epidemiology and Microbiology, USSR Academy of Medical Sciences, Moscow.
Zentralbl Bakteriol Mikrobiol Hyg A. 1988 Aug;269(2):147-55. doi: 10.1016/s0176-6724(88)80090-7.
It has been the purpose of this paper to study molecular-biological features of the Bordetella bacteriophage interaction with the host cell during lysogeny and conversion as well as to determine the degree of homology between genomes of homologous and heterologous bacteriophages. Genomes of bacteriophages from B. pertussis 134, 41405 and B. bronchiseptica 214 were studied. Heteroduplex and restriction analyses revealed a heterogeneity of bacteriophage populations, and their DNAs were found to differ in size and position of inserts. As shown by blot hybridization, the bacteriophage genome is not inserted into the chromosome of the lysogenic cell but apparently exists as an autonomous plasmid replicon. It has been established that during conversion only a part of the phage genome is inserted into the chromosome of the recipient cell.
本文旨在研究博德特氏菌噬菌体在溶原性和转化过程中与宿主细胞相互作用的分子生物学特征，以及确定同源和异源噬菌体基因组之间的同源程度。对来自百日咳博德特氏菌134、41405和支气管败血博德特氏菌214的噬菌体基因组进行了研究。异源双链分析和限制性分析揭示了噬菌体群体的异质性，并且发现它们的DNA在插入片段的大小和位置上存在差异。印迹杂交显示，噬菌体基因组并未插入溶原性细胞的染色体中，而是显然作为自主质粒复制子存在。已经确定，在转化过程中，只有噬菌体基因组的一部分插入受体细胞的染色体中。
