Lapaeva I A, Mebel' S M, Pereverzev N A, Siniashina L N
Zh Mikrobiol Epidemiol Immunobiol. 1980 May(5):85-90.
For the first time Bordetella pertussis bacteriophage was isolated, and its presence was confirmed by electron microscopy and by agar layer titration. The lysogenic strains were activated by their treatment with mitomycin C in a dose of 4.5 mg/ml. The phage system of the Bordetella genus, heretofore unknown, has been revealed: Bordetella pertussis phage lyzed all the tested strains of Bordetella parapertussis (25 strains) and could be passaged in these strains. The phage formed turbid and transparent negative colonies 0.1 mm and 0.15 mm in size. The phage titer (e. g., in strain No. 3865) was 1 X 10(10). The lysogenic variants of Bordetella pertussis, capable of spontaneous release of the phage, were obtained. These variants were characterized by changes in some of their phenotypical properties, e.g., the increased content of certain toxic substances and increased virulence.
首次分离出百日咳博德特氏菌噬菌体，并通过电子显微镜和琼脂层滴定法证实了其存在。用剂量为4.5毫克/毫升的丝裂霉素C处理溶原性菌株，使其活化。此前未知的博德特氏菌属噬菌体系统已被揭示：百日咳博德特氏菌噬菌体可裂解所有测试的副百日咳博德特氏菌菌株（25株），并可在这些菌株中传代。该噬菌体形成了大小为0.1毫米和0.15毫米的浑浊和透明阴性菌落。噬菌体效价（如在3865号菌株中）为1×10(10)。获得了能够自发释放噬菌体的百日咳博德特氏菌溶原性变体。这些变体的某些表型特性发生了变化，例如某些有毒物质含量增加和毒力增强。
