Ingelman-Sundberg M, Johansson I, Penttilä K E, Glaumann H, Lindros K O
Department of Physiological Chemistry, Karolinska Institute, Stockholm, Sweden.
Biochem Biophys Res Commun. 1988 Nov 30;157(1):55-60. doi: 10.1016/s0006-291x(88)80010-x.
Western blot analysis of digitonin eluates as well as immunohistochemical analysis revealed a 30-fold higher concentration of cytochrome P-450IIE1 in the centrilobular than in the periportal regions of the rat liver. Ethanol treatment caused a selective centrilobular induction of P-450IIE1, whereas phenobarbital induced P-450IIB1/2 in both liver lobule regions. The heterogeneous distribution pattern of P-450IIE1 was also observed in cells isolated from either region and correlated to the relative content of P-450IIE1 mRNA in the two cell types. The regiospecific expression and induction of P-450IIE1 may explain why several hepatotoxins, known to be metabolized by this isozyme, primarily damage the centrilobular region in the liver.
对洋地黄皂苷洗脱液的蛋白质免疫印迹分析以及免疫组织化学分析显示，大鼠肝脏中央小叶区域细胞色素P - 450IIE1的浓度比门周区域高30倍。乙醇处理导致中央小叶区域选择性诱导P - 450IIE1，而苯巴比妥在两个肝小叶区域均诱导P - 450IIB1/2。在从任一区域分离的细胞中也观察到P - 450IIE1的异质分布模式，并且与两种细胞类型中P - 450IIE1 mRNA的相对含量相关。P - 450IIE1的区域特异性表达和诱导可能解释了为什么几种已知由该同工酶代谢的肝毒素主要损伤肝脏的中央小叶区域。
