Jessus C, Thibier C, Huchon D, Ozon R
Laboratoire de Physiologie de la Reproduction, INRA-CNRS UA 555, Université Pierre et Marie Curie, Paris, France.
Cell Differ Dev. 1988 Sep;25(1):57-63. doi: 10.1016/0922-3371(88)90055-x.
A binding colchicine assay together with an immunostaining study with an anti-tubulin antibody showed that taxol, when added to the incubation medium, induces the formation of new microtubules in the Xenopus oocyte cortex. The capacity of the tubulin assembly in the submembranous cytoplasm decreases in progesterone-matured oocytes. In contrast, in enucleated matured oocytes this change does not occur. Altogether, these results show that taxol provokes tubulin assembly exclusively in the cortex of prophase oocyte, whereas in normal matured oocytes both cortical and cytoplasmic cytaster microtubules can be induced by taxol. The swelling of the oocyte nucleus therefore controls the spatial distribution of nucleation centers for tubulin assembly during meiotic maturation.
一项秋水仙碱结合试验以及一项使用抗微管蛋白抗体的免疫染色研究表明，当将紫杉醇添加到孵育培养基中时，它会诱导非洲爪蟾卵母细胞皮质中形成新的微管。在孕酮成熟的卵母细胞中，膜下细胞质中微管蛋白组装的能力会下降。相比之下，在去核的成熟卵母细胞中，这种变化不会发生。总之，这些结果表明，紫杉醇仅在前期卵母细胞的皮质中引发微管蛋白组装，而在正常成熟的卵母细胞中，紫杉醇可诱导皮质和细胞质星体微管的形成。因此，卵母细胞核的肿胀控制了减数分裂成熟过程中微管蛋白组装成核中心的空间分布。
