非洲爪蟾卵母细胞中微管相关蛋白（MAPs）的鉴定。
Identification of microtubule-associated proteins (MAPs) in Xenopus oocyte.
作者信息
Jessus C, Thibier C, Ozon R
出版信息
FEBS Lett. 1985 Nov 11;192(1):135-40. doi: 10.1016/0014-5793(85)80059-4.
PMID:2865168
Abstract
Microtubules were isolated from prophase-blocked oocytes of Xenopus laevis with the use of the anti-tumor drug taxol. In addition to tubulin, 5 microtubule-associated proteins (MAPs) were characterized. Among them, 2 high molecular mass proteins (200-300 kDa) are phosphorylated in ovo. The oocyte MAP extract promotes the assembly of rat brain 6 S purified tubulin.
摘要
利用抗肿瘤药物紫杉醇从非洲爪蟾前期阻滞的卵母细胞中分离出微管。除微管蛋白外，还鉴定出了5种微管相关蛋白（MAPs）。其中，有2种高分子量蛋白（200 - 300 kDa）在卵内发生磷酸化。卵母细胞MAP提取物可促进大鼠脑6S纯化微管蛋白的组装。
Zotero 插件