用于测量酵母Ⅲ号染色体稳定性的A类伪造检测法

The A-Like Faker Assay for Measuring Yeast Chromosome III Stability.

作者信息

Novoa Carolina A, Ang J Sidney, Stirling Peter C

机构信息

Terry Fox Laboratory, BC Cancer Agency, 675 W. 10th Ave, Vancouver, Canada.

Michael Smith Laboratories, University of British Columbia, 2185 East Mall, Vancouver, Canada.

出版信息

Methods Mol Biol. 2018;1672:1-9. doi: 10.1007/978-1-4939-7306-4_1.

DOI:10.1007/978-1-4939-7306-4_1

PMID:29043612

Abstract

The ability to rapidly assess chromosome instability (CIN) has enabled profiling of most yeast genes for potential effects on genome stability. The A-like faker (ALF) assay is one of several qualitative and quantitative marker loss assays that indirectly measure loss or conversion of genetic material using a counterselection step. The ALF assay relies on the ability to count spurious mating events that occur upon loss of the MATα locus of haploid Saccharomyces cerevisiae strains. Here, we describe the deployment of the ALF assay for both rapid and simple qualitative, and more in-depth quantitative analysis allowing determination of absolute ALF frequencies.

摘要

快速评估染色体不稳定性（CIN）的能力使得能够对大多数酵母基因进行分析，以研究其对基因组稳定性的潜在影响。A类伪造者（ALF）检测是几种定性和定量标记丢失检测方法之一，这些方法通过反选择步骤间接测量遗传物质的丢失或转换。ALF检测依赖于对单倍体酿酒酵母菌株MATα位点丢失时发生的假交配事件进行计数的能力。在此，我们描述了ALF检测的应用，它既可以进行快速简单的定性分析，也可以进行更深入的定量分析，从而确定绝对ALF频率。