• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

细胞中 DNA 修复内切酶缺失的成分的化学-遗传相互作用似乎与液泡破坏有关。

Chemical-Genetic Interactions of Constituents in Cells Deficient for the DNA Repair Endonuclease Appear Linked to Vacuolar Disruption.

机构信息

Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.

Toxicology Graduate Program, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.

出版信息

Molecules. 2021 Feb 24;26(5):1207. doi: 10.3390/molecules26051207.

DOI:10.3390/molecules26051207
PMID:33668176
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7956252/
Abstract

Colorectal cancer is a common cancer worldwide and reduced expression of the DNA repair endonuclease XPF (xeroderma pigmentosum complementation group F) is associated with colorectal cancer. extracts were previously found to exhibit chemical-genetic synthetic lethal effects in a model of colorectal cancer lacking Rad1p, a structural and functional homologue of human XPF. However, the mechanisms for extracts to limit proliferation and promote an apoptosis-like event in deleted yeast was not elucidated. Our current analysis has revealed that extracts have the capacity to promote mutations in ∆ cells. In addition, the effects of extracts on ∆ yeast is linked to disruption of the vacuole, similar to the mammalian lysosome. The absence of in yeast sensitizes cells to the effects of vacuole disruption and the release of proteases. The combined effect of increased DNA mutations and release of vacuolar contents appears to induce an apoptosis-like event that is dependent on the meta-caspase Yca1p. The toxicity of extracts is linked to sterol content, suggesting saponins may be involved in limiting the proliferation of yeast cells. Analysis of major constituents from identified a chemical-genetic interaction between bacopasaponin C and ∆ yeast. Bacopasaponin C may have potential as a drug candidate or serve as a model for the development of analogs for the treatment of colorectal cancer.

摘要

结直肠癌是一种常见的癌症，DNA 修复内切酶 XPF（着色性干皮病互补组 F）表达降低与结直肠癌有关。先前发现，在缺乏 Rad1p（人类 XPF 的结构和功能同源物）的结直肠癌细胞模型中， 提取物表现出化学遗传合成致死效应。然而， 提取物在缺失酵母中限制增殖并促进类似凋亡事件的机制尚未阐明。我们目前的分析表明， 提取物具有促进 ∆ 细胞突变的能力。此外， 提取物对 ∆ 酵母的影响与液泡的破坏有关，类似于哺乳动物溶酶体。酵母中 的缺失使细胞对液泡破坏和蛋白酶释放的影响敏感。增加的 DNA 突变和液泡内容物的释放的联合作用似乎诱导了依赖于元半胱天冬酶 Yca1p 的类似凋亡的事件。 提取物的毒性与固醇含量有关，表明皂苷可能参与限制酵母细胞的增殖。对 中的主要成分的分析确定了 Bacopasaponin C 与 ∆ 酵母之间的化学遗传相互作用。Bacopasaponin C 可能具有作为药物候选物的潜力，或作为开发用于治疗结直肠癌的类似物的模型。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a034/7956252/693629f8509e/molecules-26-01207-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a034/7956252/34b6e6ba8ecb/molecules-26-01207-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a034/7956252/daf88ef7e918/molecules-26-01207-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a034/7956252/53a09474cdee/molecules-26-01207-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a034/7956252/1418de6c672d/molecules-26-01207-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a034/7956252/17db13c4c84f/molecules-26-01207-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a034/7956252/de41b69d09b8/molecules-26-01207-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a034/7956252/bcfbc6377af0/molecules-26-01207-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a034/7956252/b943cf3087a1/molecules-26-01207-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a034/7956252/e335fc78c656/molecules-26-01207-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a034/7956252/4784741d3be9/molecules-26-01207-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a034/7956252/693629f8509e/molecules-26-01207-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a034/7956252/34b6e6ba8ecb/molecules-26-01207-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a034/7956252/daf88ef7e918/molecules-26-01207-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a034/7956252/53a09474cdee/molecules-26-01207-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a034/7956252/1418de6c672d/molecules-26-01207-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a034/7956252/17db13c4c84f/molecules-26-01207-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a034/7956252/de41b69d09b8/molecules-26-01207-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a034/7956252/bcfbc6377af0/molecules-26-01207-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a034/7956252/b943cf3087a1/molecules-26-01207-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a034/7956252/e335fc78c656/molecules-26-01207-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a034/7956252/4784741d3be9/molecules-26-01207-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a034/7956252/693629f8509e/molecules-26-01207-g011.jpg

相似文献

1
Chemical-Genetic Interactions of Constituents in Cells Deficient for the DNA Repair Endonuclease Appear Linked to Vacuolar Disruption.细胞中 DNA 修复内切酶缺失的成分的化学-遗传相互作用似乎与液泡破坏有关。
Molecules. 2021 Feb 24;26(5):1207. doi: 10.3390/molecules26051207.
2
Interrogation of ethnomedicinal plants for synthetic lethality effects in combination with deficiency in the DNA repair endonuclease RAD1 using a yeast cell-based assay.采用基于酵母细胞的测定法，从民族医学植物中筛选与 DNA 修复内切酶 RAD1 缺乏相组合的合成致死效应。
J Ethnopharmacol. 2018 Sep 15;223:10-21. doi: 10.1016/j.jep.2018.05.016. Epub 2018 May 17.
3
Two new triterpenoid glycosides from and their cytotoxic activity.从 中分离得到的两种新三萜糖苷及其细胞毒性。
Nat Prod Res. 2024 Apr;38(7):1120-1126. doi: 10.1080/14786419.2022.2132498. Epub 2022 Oct 14.
4
Estimation of twelve bacopa saponins in Bacopa monnieri extracts and formulations by high-performance liquid chromatography.采用高效液相色谱法测定积雪草提取物及制剂中12种积雪草皂苷的含量。
Chem Pharm Bull (Tokyo). 2006 Jun;54(6):907-11. doi: 10.1248/cpb.54.907.
5
Quantitative determination of the major saponin mixture bacoside A in Bacopa monnieri by HPLC.采用高效液相色谱法对积雪草中的主要皂苷混合物积雪草苷A进行定量测定。
Phytochem Anal. 2005 Jan-Feb;16(1):24-9. doi: 10.1002/pca.805.
6
Distinct roles of XPF-ERCC1 and Rad1-Rad10-Saw1 in replication-coupled and uncoupled inter-strand crosslink repair.XPF-ERCC1 和 Rad1-Rad10-Saw1 在复制偶联和非偶联的链间交联修复中具有不同的作用。
Nat Commun. 2018 May 23;9(1):2025. doi: 10.1038/s41467-018-04327-0.
7
Attenuation of cisplatin-induced emetogenesis by standardized Bacopa monnieri extracts in the pigeon: behavioral and neurochemical correlations.标准化积雪草提取物对顺铂诱导的鸽子呕吐反应的减轻作用：行为学与神经化学相关性
Planta Med. 2014 Nov;80(17):1569-79. doi: 10.1055/s-0034-1383121. Epub 2014 Oct 8.
8
Triterpenoid glycosides from Bacopa monnieri.来自假马齿苋的三萜类糖苷。
Phytochemistry. 2005 Dec;66(23):2719-28. doi: 10.1016/j.phytochem.2005.09.016. Epub 2005 Nov 15.
9
LC-ESI-QTOF-MS based screening and identification of isomeric jujubogenin and pseudojujubogenin aglycones in Bacopa monnieri extract.基于液相色谱-电喷雾电离-四极杆飞行时间质谱法筛选与鉴定积雪草提取物中的异构化酸枣仁皂苷元及伪酸枣仁皂苷元苷元。
J Pharm Biomed Anal. 2016 Sep 10;129:121-134. doi: 10.1016/j.jpba.2016.06.052. Epub 2016 Jun 29.
10
'Bacoside B'--the need remains for establishing identity.'Bacoside B'--确定其身份仍有必要。
Fitoterapia. 2013 Jun;87:7-10. doi: 10.1016/j.fitote.2013.03.011. Epub 2013 Mar 16.

引用本文的文献

1
Pharmacological attributes of extract: Current updates and clinical manifestation.提取物的药理学特性：最新进展与临床表现
Front Nutr. 2022 Aug 18;9:972379. doi: 10.3389/fnut.2022.972379. eCollection 2022.
2
Bacopa Protects against Neurotoxicity Induced by MPP and Methamphetamine.Bacopa 可预防 MPP+ 和甲基苯丙胺引起的神经毒性。
Molecules. 2022 Aug 15;27(16):5204. doi: 10.3390/molecules27165204.
3
Mechanisms of Genome Maintenance in Plants: Playing It Safe With Breaks and Bumps.植物基因组维持的机制：在断裂与碰撞中确保安全

本文引用的文献

1
Interrogation of ethnomedicinal plants for synthetic lethality effects in combination with deficiency in the DNA repair endonuclease RAD1 using a yeast cell-based assay.采用基于酵母细胞的测定法，从民族医学植物中筛选与 DNA 修复内切酶 RAD1 缺乏相组合的合成致死效应。
J Ethnopharmacol. 2018 Sep 15;223:10-21. doi: 10.1016/j.jep.2018.05.016. Epub 2018 May 17.
2
The A-Like Faker Assay for Measuring Yeast Chromosome III Stability.用于测量酵母Ⅲ号染色体稳定性的A类伪造检测法
Methods Mol Biol. 2018;1672:1-9. doi: 10.1007/978-1-4939-7306-4_1.
3
Cancer Statistics, 2017.
Front Genet. 2021 Jun 22;12:675686. doi: 10.3389/fgene.2021.675686. eCollection 2021.
《2017 年癌症统计》
CA Cancer J Clin. 2017 Jan;67(1):7-30. doi: 10.3322/caac.21387. Epub 2017 Jan 5.
4
Development of Ayurveda - Tradition to trend.阿育吠陀的发展——传统到潮流。
J Ethnopharmacol. 2017 Feb 2;197:10-24. doi: 10.1016/j.jep.2016.09.024. Epub 2016 Sep 12.
5
Differential Inhibition of Water and Ion Channel Activities of Mammalian Aquaporin-1 by Two Structurally Related Bacopaside Compounds Derived from the Medicinal Plant Bacopa monnieri.两种源自药用植物假马齿苋的结构相关的假马齿苋皂苷化合物对哺乳动物水通道蛋白-1水和离子通道活性的差异抑制作用
Mol Pharmacol. 2016 Oct;90(4):496-507. doi: 10.1124/mol.116.105882. Epub 2016 Jul 29.
6
External and internal triggers of cell death in yeast.酵母细胞死亡的外部和内部触发因素。
Cell Mol Life Sci. 2016 Jun;73(11-12):2237-50. doi: 10.1007/s00018-016-2197-y. Epub 2016 Apr 5.
7
Evaluation of anticancer potential of Bacopa monnieri L. against MCF-7 and MDA-MB 231 cell line.水飞蓟宾对MCF - 7和MDA - MB 231细胞系的抗癌潜力评估。 （注：你提供的原文中Bacopa monnieri L.常见中文名是假马齿苋，这里按照你要求的准确翻译，但感觉可能原文有误，正常应该是Bacopaside Ⅱ中文名水飞蓟宾，供参考。）
J Pharm Bioallied Sci. 2015 Oct-Dec;7(4):325-8. doi: 10.4103/0975-7406.168038.
8
In Silico and In Vitro Analysis of Bacoside A Aglycones and Its Derivatives as the Constituents Responsible for the Cognitive Effects of Bacopa monnieri.积雪草苷苷元及其衍生物作为积雪草认知效应相关成分的计算机模拟和体外分析
PLoS One. 2015 May 12;10(5):e0126565. doi: 10.1371/journal.pone.0126565. eCollection 2015.
9
The amphiphilic nature of saponins and their effects on artificial and biological membranes and potential consequences for red blood and cancer cells.皂苷的两亲性质及其对人工和生物膜的影响，以及对红细胞和癌细胞的潜在后果。
Org Biomol Chem. 2014 Nov 28;12(44):8803-22. doi: 10.1039/c4ob01652a.
10
Cancer incidence and mortality worldwide: sources, methods and major patterns in GLOBOCAN 2012.全球癌症发病与死亡：GLOBOCAN 2012 数据源、方法与主要模式。
Int J Cancer. 2015 Mar 1;136(5):E359-86. doi: 10.1002/ijc.29210. Epub 2014 Oct 9.