用于光学显微镜和荧光显微镜的内质网标记

Labeling the ER for Light and Fluorescence Microscopy.

作者信息

Hawes Chris, Wang Pengwei, Kriechbaumer Verena

机构信息

Department of Biological and Medical Sciences, Oxford Brookes University, OX3 0BP, Oxford, UK.

Key Laboratory of Horticultural Plant Biology (MOE), College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, Hubei Province, PR China.

出版信息

Methods Mol Biol. 2018;1691:1-14. doi: 10.1007/978-1-4939-7389-7_1.

DOI:10.1007/978-1-4939-7389-7_1

PMID:29043665

Abstract

The ER is a highly dynamic network of tubules and membrane sheets. Hence imaging this organelle in its native and mobile state is of great importance. Here we describe methods of labeling the native ER using fluorescent proteins and lipid dyes as well as methods for immunolabeling on plant tissue.

摘要

内质网是由小管和膜片组成的高度动态的网络。因此，对处于天然和移动状态的这种细胞器进行成像非常重要。在这里，我们描述了使用荧光蛋白和脂质染料标记天然内质网的方法，以及在植物组织上进行免疫标记的方法。