让它发光：为明场和荧光显微镜标记 ER。
Make It Shine: Labelling the ER for Light and Fluorescence Microscopy.
机构信息
Endomembrane Structure and Function Research Group, Department of Biological and Medical Sciences, Oxford Brookes University, Oxford, UK.
College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, Hubei Province, China.
出版信息
Methods Mol Biol. 2024;2772:1-14. doi: 10.1007/978-1-0716-3710-4_1.
PMID:38411802
Abstract
The ER is a highly dynamic network of tubules and membrane cisternae. Hence, imaging this organelle in its native and mobile state is of great importance. Here we describe methods of labelling the native plant ER using fluorescent proteins and lipid dyes as well as methods for immunolabelling on plant tissue.
摘要
内质网是一个高度动态的管状和膜腔网络。因此，在其天然和运动状态下对该细胞器进行成像具有重要意义。在这里，我们描述了使用荧光蛋白和脂质染料对天然植物内质网进行标记的方法，以及对植物组织进行免疫标记的方法。
Zotero 插件