TCM Clinical Basis Institute, Zhejiang Chinese Medicine University, 548 Binwen Road, Hangzhou, Zhejiang, 310000, China.
Guangzhou University of Chinese Medicine, Mathematical Engineering Academy of Chinese Medicine, Guangzhou, 510006, China.
Sci Rep. 2017 Oct 18;7(1):13436. doi: 10.1038/s41598-017-13723-3.
Traditional Chinese Medicine (TCM) has been served as complementary medicine for Ankylosing Spondylitis (AS) treatment for a long time. Yun-Pi-Yi-Shen-Tong-Du-Tang (Y-Y-T) is a novel empirical formula designed by Prof. Chengping Wen. In this study, a retrospective investigation supported efficacy of Y-Y-T and then we deciphered the underlying molecular mechanism of the efficacy. Herbal ingredients and targeting proteins were collected from TCMID. PPI networks were constructed to further infer the relationship among Y-Y-T, drugs used for treating AS, differentially expressed genes of AS patients and AS disease proteins. Finally, it was suggested that TLR signaling pathway and T cell receptor signaling pathway may involve in the biological processes of AS progression and contribute to the curative effect and proteins such as JAK2, STAT3, HSP90AA1, TNF and PTEN were the key targets. Our systemic investigation to infer therapeutic mechanism of Y-Y-T for AS treatment provides a new insight in understanding TCM pharmacology.
中药（TCM）长期以来一直作为强直性脊柱炎（AS）治疗的辅助药物。运脾益肾通督汤（Y-Y-T）是温成平教授设计的一种新的经验方。本研究通过回顾性调查支持了 Y-Y-T 的疗效，然后我们破译了疗效的潜在分子机制。草药成分和靶向蛋白从 TCMID 中收集。构建 PPI 网络，以进一步推断 Y-Y-T、用于治疗 AS 的药物、AS 患者差异表达基因和 AS 疾病蛋白之间的关系。最后，提示 TLR 信号通路和 T 细胞受体信号通路可能参与 AS 进展的生物学过程，并有助于疗效，JAK2、STAT3、HSP90AA1、TNF 和 PTEN 等蛋白是关键靶点。我们对 Y-Y-T 治疗 AS 的治疗机制进行系统推断的研究，为理解中医药理学提供了新的视角。
