Xu Chunping, Yu Jinwei, Zhao Shanshan, Wu Shungshung, He Peixin, Jia Xuewei, Liu Yanqi, Mao Duobin
College of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou, 450002 Henan, P.R. China.
Collaborative Innovation Center of Food Production and Safety, Zhengzhou, 450002 Henan, P.R. China.
An Acad Bras Cienc. 2017;89(3 Suppl):2033-2041. doi: 10.1590/0001-3765201720150786. Epub 2017 Oct 16.
The effect on different three carbon source (i.e. glucose, fructose and sucrose) on production, chemical characterization and antioxidant activity of exopolysaccharide (EPS) produced by Phellinus vaninii Ljup was investigated in this study. Amongst carbon sources examined, glucose and sucrose were favorable for the mycelia growth, while the maximum EPS yield was achieved when sucrose was employed. The predominant carbohydrate compositions in EPSs identified were gluconic acid, glucose, mannose and galactose acid. Then, FT-IR spectral analysis revealed prominent characteristic groups in EPSs. EPSs molecule exist as nearly globular shape form in aqueous solution. The variation also affects antioxidant activities by investigated by using hydroxyl and DPPH radical scavenging assay. Sucrose was best carbon source from the viewpoint of antioxidant activity due to the relatively high contents of galactose in the EPS with moderate molecular weight and polydispersity.
本研究考察了不同三种碳源（即葡萄糖、果糖和蔗糖）对瓦尼拟层孔菌（Phellinus vaninii Ljup）产生的胞外多糖（EPS）产量、化学特性及抗氧化活性的影响。在所考察的碳源中，葡萄糖和蔗糖有利于菌丝体生长，而使用蔗糖时EPS产量最高。鉴定出的EPS中主要的碳水化合物成分是葡萄糖酸、葡萄糖、甘露糖和半乳糖酸。然后，傅里叶变换红外光谱分析揭示了EPS中的显著特征基团。EPS分子在水溶液中以近似球形的形式存在。通过羟自由基和DPPH自由基清除试验研究发现，这种变化也会影响抗氧化活性。从抗氧化活性的角度来看，蔗糖是最佳碳源，因为其EPS中半乳糖含量相对较高，且分子量和多分散性适中。
