Zhuansun Wanwan, Xu Jun, Liu Hengzhao, Zhao Ying, Chen Lulu, Shan Shufang, Song Shiqin, Zhang Haoyu, Dong Tingting, Zeng Huawei, Xu Qinxiang
Anhui Province Key Laboratory of Pollutant Sensitive Materials and Environmental Remediation, School of Life Sciences Huaibei Normal University, Huaibei, China.
R&D Center of Anhui Kouzi Distillery Co., Ltd, Huaibei, China.
Front Nutr. 2022 Oct 20;9:1032289. doi: 10.3389/fnut.2022.1032289. eCollection 2022.
The fermentation medium of a newly identified was optimized by response surface methodology, with the optimal medium containing sucrose (80 g/L), yeast powder (60 g/L), KHPO (5 g/L), MgSO·7HO (1 g/L) and NaSeO (0. 1 g/L). Under these conditions, the extracellular polysaccharide yield was 8.09 g/L. A novel selenium-enriched polysaccharide (PACI-1) was isolated from , purified and identified as a homofructose polysaccharide with a low average molecular weight of 9.95 × 10 Da. The fine structure of PACI-1 was analyzed using NMR, CD, and AFM. Additionally, the antioxidant results showed that the PACI-1 had stronger antioxidant capacity than natural polysaccharides. These results provided a candidate strain for producing selenium polysaccharide and a new polysaccharide from , which showed good antioxidant activity.
采用响应面法对新鉴定的[微生物名称未给出]发酵培养基进行优化，优化后的培养基含有蔗糖（80 g/L）、酵母粉（60 g/L）、KH₂PO₄（5 g/L）、MgSO₄·7H₂O（1 g/L）和Na₂SeO₃（0.1 g/L）。在此条件下，胞外多糖产量为8.09 g/L。从[微生物名称未给出]中分离出一种新型富硒多糖（PACI - 1），经纯化并鉴定为平均分子量较低的9.95×10³ Da的同型果糖多糖。利用核磁共振（NMR）、圆二色光谱（CD）和原子力显微镜（AFM）对PACI - 1的精细结构进行了分析。此外，抗氧化结果表明PACI - 1具有比天然多糖更强的抗氧化能力。这些结果为生产硒多糖提供了候选菌株，并从[微生物名称未给出]中得到了一种具有良好抗氧化活性的新多糖。
