Department of Dermatology, Ajou University School of Medicine, Suwon, Korea.
Department of Dermatology, Seoul Medical Center, Seoul, Korea.
Pigment Cell Melanoma Res. 2018 Mar;31(2):277-286. doi: 10.1111/pcmr.12658. Epub 2017 Nov 10.
Photodynamic therapy (PDT) is a treatment option for skin cancer and premalignant skin diseases and exhibits rejuvenation effects, including reducing fine wrinkles and whitening, on aged skin. In this study, we investigated the mechanism underlying the whitening effects of PDT on melanocytes (MCs) in vitro and in vivo. Exposure of MCs to PDT in vitro reduced their melanin content and tyrosinase activity without, however, affecting cell survival. Interestingly, melanogenesis was also inhibited by exposing MCs to conditioned media of PDT-treated keratinocytes or dermal fibroblasts. This paracrine effect was likely due to a decreased release of melanocyte-stimulating cytokines such as Kit ligand and hepatocyte growth factor from these cells. Furthermore, we observed that PDT reduced mottled hyperpigmentation of photoaged patient skin in vivo, highlighting the clinical importance of skin whitening by PDT.
光动力疗法 (PDT) 是一种治疗皮肤癌和癌前皮肤疾病的方法，并且对老年皮肤具有年轻化效果，包括减少细小皱纹和美白。在这项研究中，我们研究了 PDT 对体外和体内黑素细胞 (MC) 的美白作用的机制。体外 PDT 暴露降低了 MC 的黑色素含量和酪氨酸酶活性，而不影响细胞存活。有趣的是，MC 暴露于 PDT 处理的角质形成细胞或真皮成纤维细胞的条件培养基也抑制了黑素生成。这种旁分泌作用可能是由于这些细胞中黑素细胞刺激细胞因子（如 Kit 配体和肝细胞生长因子）的释放减少所致。此外，我们观察到 PDT 减少了光老化患者皮肤的斑驳性色素沉着过度，突出了 PDT 皮肤美白的临床重要性。
