Li Junjian, Zuo Gancheng, Pan Xihao, Wei Wei, Qi Xiaoliang, Su Ting, Dong Wei
School of Chemical Engineering, Nanjing University of Science and Technology, Nanjing, People's Republic of China.
Luminescence. 2018 Feb;33(1):243-248. doi: 10.1002/bio.3407. Epub 2017 Oct 18.
An easy hydrothermal synthesis strategy was applied to synthesize green-yellow emitting nitrogen-doped carbon dots (N-CDs) using 1,2-diaminobenzene as the carbon source, and dicyandiamide as the dopant. The nitrogen-doped CDs resulted in improvement in the electronic characteristics and surface chemical activities. N-CDs exhibited bright fluorescence emission and could response to Ag selectively and sensitively. Other ions produced nearly no interference. A N-CDs based fluorescent probe was then applied to sensitively determine Ag with a detection limit of 5 × 10 mol/L. The method was applied to the determination of Ag dissolved in water. Finally, negligibly cytotoxic, excellently biocompatibile, and highly fluorescent carbon dots were applied for HepG2 cell imaging and the quenched fluorescence by adding Ag , which indicated its potential applications.
采用一种简单的水热合成策略，以1,2 - 二氨基苯为碳源、双氰胺为掺杂剂，合成了发出绿黄色荧光的氮掺杂碳点（N - CDs）。氮掺杂碳点改善了电子特性和表面化学活性。N - CDs表现出明亮的荧光发射，并且能够对Ag进行选择性和灵敏的响应。其他离子几乎不产生干扰。然后将基于N - CDs的荧光探针用于灵敏地测定Ag，检测限为5×10⁻⁸ mol/L。该方法应用于水中溶解Ag的测定。最后，将具有可忽略的细胞毒性、优异的生物相容性和高荧光性的碳点用于HepG2细胞成像，通过添加Ag使荧光猝灭，这表明了其潜在应用。
