基于新鲜茶叶的氮掺杂碳点的绿色合成用于选择性铁离子检测及细胞成像

Green Synthesis of Nitrogen-Doped Carbon Dots from Fresh Tea Leaves for Selective Fe Ions Detection and Cellular Imaging.

作者信息

Ge Guili, Li Lin, Chen Mingjian, Wu Xu, Yang Yuxin, Wang Dan, Zuo Sicheng, Zeng Zhaoyang, Xiong Wei, Guo Can

机构信息

Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute and School of Basic Medical Science, Central South University, Changsha 410008, China.

College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.

出版信息

Nanomaterials (Basel). 2022 Mar 17;12(6):986. doi: 10.3390/nano12060986.

In this research, we successfully developed a green, economical and effective one-step hydrothermal method for the synthesis of fluorescent nitrogen-doped carbon dots (N-CDs) by utilizing fresh tea leaves and urea as the carbon and nitrogen sources, respectively. The obtained N-CDs were characterized by TEM, XPS and FT-IR. We found that the N-CDs were near-spherical with an average size of about 2.32 nm, and contained abundant oxygen and nitrogen functional groups. The N-CDs exhibited bright blue fluorescence under ultraviolet illumination, with the maximum emission at 455 nm. Meanwhile, the as-prepared N-CDs could be selectively quenched by Fe ions. The quenching of N-CDs is linearly correlated with the concentration of Fe in the range of 0.1-400 μM with a low detection limit of 0.079 μM. Significantly, the N-CDs present excellent biocompatibility and high photostability. The results also depict that multicolor fluorescence is displayed under a fluorescence microscope and successfully applied for the detection of intracellular Fe. To sum up, the fluorescent N-CDs are expected to be a sensitive detection probe for Fe in biological systems.

在本研究中，我们成功开发了一种绿色、经济且高效的一步水热法，通过分别利用新鲜茶叶和尿素作为碳源和氮源来合成荧光氮掺杂碳点（N-CDs）。所制备的N-CDs通过透射电子显微镜（TEM）、X射线光电子能谱（XPS）和傅里叶变换红外光谱（FT-IR）进行表征。我们发现N-CDs呈近球形，平均尺寸约为2.32 nm，且含有丰富的氧和氮官能团。N-CDs在紫外光照射下呈现明亮的蓝色荧光，最大发射波长为455 nm。同时，所制备的N-CDs能被铁离子选择性猝灭。在0.1 - 400 μM范围内，N-CDs的猝灭与铁的浓度呈线性相关，检测限低至0.079 μM。值得注意的是，N-CDs具有优异的生物相容性和高光稳定性。结果还表明，在荧光显微镜下可呈现多色荧光，并成功应用于细胞内铁的检测。综上所述，荧光N-CDs有望成为生物体系中铁的灵敏检测探针。