Ghosh Amlan, Dutta Shampa, Podder Sanjoy, Mondal Priti, Laha Arghya, Saha Nimai Chandra, Moitra Saibal, Saha Goutam Kumar
Department of Life Sciences, Presidency University, Kolkata, West Bengal, India.
Post Graduate Department of Zoology, Barasat Government College, Kolkata, India.
J Med Entomol. 2018 Jan 10;55(1):14-19. doi: 10.1093/jme/tjx178.
India is the home to around 15-20 million asthmatics, and asthma prevalence is increasing in Indian metropolitan area, including Kolkata, West Bengal. Complex interactions of genetic and environmental factors are involved in asthma. Genome-wide search for susceptible loci regulating IgE response (atopy) have identified a candidate gene CD14 which is most important in the context of allergic responses of respiratory system. This study was aimed to investigate the role of house dust and house dust mites in development of bronchial asthma and to explore the possible association of candidate gene CD14 with disease manifestation among Kolkata patient population. Skin-prick test was done among 950 asthmatic patients against 8 aeroallergens, including house dust and house dust mites and total serum IgE and allergen-specific IgE were measured. Polymerase chain reaction-restriction fragment length polymorphism was done in patients and nonasthmatic control (n = 255 in each) to characterize a functional polymorphism, C(-159)T, of CD14, a positional candidate gene for allergy. We identified house dust as the most common aeroallergen sensitizer among atopic patients in Kolkata followed by Dermatophagoides pteronyssinus and Dermatophagoides farinae Hughes (Acari: Pyroglyphidae) mites. Patient's sera contain significantly higher IgE level than that of control. Allergen-specific IgE antibody test revealed that 76.36% patients had specific IgE antibody against D. pteronyssinus mite. There was a significant difference in the distribution of alleles and genotypes for CD14 polymorphism with an increase in disease severity. So, in Kolkata, house dust mite is a common aeroallergen and D. pteronyssinus is predominant among mites. The present study revealed that bronchial asthma has a genetic background.
印度约有1500万至2000万哮喘患者，且在包括西孟加拉邦加尔各答在内的印度大都市地区，哮喘患病率正在上升。哮喘涉及遗传和环境因素的复杂相互作用。全基因组搜索调节IgE反应（特应性）的易感基因座已确定一个候选基因CD14，它在呼吸系统过敏反应中最为重要。本研究旨在调查屋尘和屋尘螨在支气管哮喘发病中的作用，并探讨候选基因CD14与加尔各答患者群体疾病表现之间的可能关联。对950名哮喘患者进行了针对8种气传变应原（包括屋尘和屋尘螨）的皮肤点刺试验，并检测了总血清IgE和变应原特异性IgE。对患者和非哮喘对照者（每组n = 255）进行聚合酶链反应-限制性片段长度多态性分析，以鉴定过敏定位候选基因CD14的功能性多态性C(-159)T。我们确定屋尘是加尔各答特应性患者中最常见的气传变应原致敏原，其次是粉尘螨和屋尘螨（蜱螨亚纲：尘螨科）。患者血清中的IgE水平显著高于对照组。变应原特异性IgE抗体检测显示，76.36%的患者具有针对粉尘螨的特异性IgE抗体。随着疾病严重程度的增加，CD14多态性的等位基因和基因型分布存在显著差异。因此，在加尔各答，屋尘螨是常见的气传变应原，且粉尘螨在螨类中占主导地位。本研究表明支气管哮喘具有遗传背景。
