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CY1的高质量基因组草图序列,具有生物修复镉、铬和硒污染的潜力。

High-quality-draft genomic sequence of CY1 with the potential to bioremediate Cd, Cr and Se contamination.

作者信息

Li Jingxin, Guo Wei, Shi Manman, Cao Yajing, Wang Gejiao

机构信息

State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070 People's Republic of China.

出版信息

Stand Genomic Sci. 2017 Oct 10;12:60. doi: 10.1186/s40793-017-0273-z. eCollection 2017.

Abstract

CY1 (= KCTC 33419 = CCTCC AB2013369) is a Gram-positive, aerobic, endospore-forming, motile and rod-shaped bacterium isolated from iron mineral soil. This bacterium reduces sulfate (SO) to S, which reacts with Cd(II) to generate precipitated CdS. It also reduces the toxic chromate [Cr(VI)] and selenite [Se(VI)] to the less bioavailable chromite [Cr(III)] and selenium (Se), respectively. Thus, strain CY1 has the potential to bioremediate Cd, Cr and Se contamination, which is the main reason for the interest in sequencing its genome. Here we describe the features of strain CY1, together with the draft genome sequence and its annotation. The 9,184,169 bp long genome exhibits a G + C content of 45.6%, 7909 protein-coding genes and 81 RNA genes. Nine putative Se(IV)-reducing genes, five putative Cr(VI) reductase and nine putative sulfate-reducing genes were identified in the genome.

摘要

CY1(=KCTC 33419 = CCTCC AB2013369)是一种革兰氏阳性、需氧、产芽孢、具运动性的杆状细菌,从铁矿物土壤中分离得到。该细菌可将硫酸根(SO)还原为硫,硫与镉(II)反应生成沉淀硫化镉。它还能分别将有毒的铬酸盐[Cr(VI)]和亚硒酸盐[Se(VI)]还原为生物可利用性较低的亚铬酸盐[Cr(III)]和硒(Se)。因此,CY1菌株具有对镉、铬和硒污染进行生物修复的潜力,这也是对其进行基因组测序的主要原因。在此,我们描述了CY1菌株的特征,以及其基因组草图序列和注释。该基因组长度为9,184,169 bp,G + C含量为45.6%,有7909个蛋白质编码基因和81个RNA基因。在基因组中鉴定出9个假定的亚硒酸盐(IV)还原基因、5个假定的铬酸盐(VI)还原酶和9个假定的硫酸盐还原基因。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e845/5634878/3e64fdd5ab47/40793_2017_273_Fig1_HTML.jpg

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