Huq Md Amdadul
Department of Food and Nutrition, College of Biotechnology and Natural Resource, Chung-Ang University, Anseong-si, Gyeonggi-do, 17546, Republic of Korea.
Curr Microbiol. 2020 Sep;77(9):2023-2030. doi: 10.1007/s00284-020-02086-0. Epub 2020 Jun 15.
A silver nanoparticle (AgNP) producing, Gram stain-positive, aerobic, motile, and rod-shaped novel bacterial strain, designated as MAH-34 was isolated from rhizospheric soil of magnolia tree. The colonies were creamy white, smooth, circular, and 0.9-2.0 mm in diameter when grown on R2A agar. Strain MAH-34 was found to be able to grow at 10-37 °C, at pH 6.0-9.5, and at 0-1% NaCl. The strain showed activity for both catalase, and oxidase tests, and was able to rapid synthesis of AgNPs. The TEM image revealed the spherical shape of biosynthesized AgNPs, and the size was 5 to 15 nm. Based on 16S rRNA gene sequence comparisons, the isolate was shown to be a member of genus Paenibacillus, and the close type strains were Paenibacillus chondroitinus DSM 5051 (98.3%), Paenibacillus aceris KUDC4121 (98.2%), Paenibacillus nebraskensis JJ-59 (97.8%), Paenibacillus alginolyticus DSM 5050 (97.6%), Paenibacillus ferrarius CY1 (97.4%), Paenibacillus frigoriresistens YIM 016 (97.3%), and Paenibacillus pocheonensis Gsoil 1138 (97.3%). Strain MAH-34 had a genome size of 8,647,010 bp. The genomic G + C content was 46.0 mol %. The major isoprenoid quinone was determined as menaquinone-7 (MK-7). The major cellular fatty acids were determined as C, and C. Based on the DNA-DNA hybridization results, genotypic analysis, chemotaxonomic, and physiological data, strain MAH-34 represents a novel species, for which the name Paenibacillus anseongense sp. nov. is proposed, with MAH-34 as the type strain (= KACC 19974 = CGMCC1.16610).
从木兰树的根际土壤中分离出一株产银纳米颗粒（AgNP）、革兰氏染色阳性、需氧、运动性强且呈杆状的新型细菌菌株，命名为MAH-34。该菌落在R2A琼脂平板上生长时呈乳白色、光滑、圆形，直径为0.9 - 2.0毫米。发现菌株MAH-34能够在10 - 37°C、pH值为6.0 - 9.5以及0 - 1% NaCl的条件下生长。该菌株在过氧化氢酶和氧化酶试验中均表现出活性，并且能够快速合成AgNP。透射电子显微镜图像显示生物合成的AgNP呈球形，尺寸为5至15纳米。基于16S rRNA基因序列比较，该分离株被证明是芽孢杆菌属的成员，与其亲缘关系较近的模式菌株有嗜软骨芽孢杆菌DSM 5051（98.3%）、槭树芽孢杆菌KUDC4121（98.2%）、内布拉斯加芽孢杆菌JJ-59（97.8%）、解藻酸芽孢杆菌DSM 5050（97.6%）、嗜铁芽孢杆菌CY1（97.4%）、耐冷芽孢杆菌YIM 016（97.3%）以及抱川芽孢杆菌Gsoil 1138（97.3%）。菌株MAH-34的基因组大小为8,647,010碱基对。基因组G + C含量为46.0摩尔%。主要的类异戊二烯醌被确定为甲基萘醌-7（MK-7）。主要的细胞脂肪酸被确定为C和C。基于DNA - DNA杂交结果、基因型分析、化学分类学和生理学数据，菌株MAH-34代表一个新物种，为此提出名为安城芽孢杆菌（Paenibacillus anseongense sp. nov.），以MAH-34作为模式菌株（= KACC 19974 = CGMCC1.16610）。
