Kim Ki Su, Kim Yun Seop, Bao Kai, Wada Hideyuki, Choi Hak Soo, Hahn Sei Kwang
PHI BIOMED Co., #613, 12 Gangnam-daero 65-gil, Seocho-gu, Seoul, 06612 South Korea.
Department of Organic Materials Science and Engineering, College of Engineering, Pusan National University, 2 Busandaehak-ro 63 beon-gil, Geumjeong-gu, Busan, 46241 South Korea.
Biomater Res. 2017 Oct 10;21:15. doi: 10.1186/s40824-017-0102-x. eCollection 2017.
The injection of botulinum toxin (BTX) to reduce facial wrinkles is one of the most frequently performed plastic surgery procedures. The biocompatible hydrogels are injected with BTX for effective tissue augmentation. However, it is difficult to determine the interval of injection for effective tissue augmentation.
BTX and hyaluronate (HA) hydrogels were labeled with zwitterionic (ZW) near-infrared (NIR) fluorophores and visualized for 3 weeks after injection to BALB/c nude mice.
BTX-ZW conjugates and diaminohexane (DAH)-HA-ZW hydrogels were successfully prepared by the conventional EDC/NHS chemistry. Using the NIR fluorescence imaging, we confirmed that approximately 10% of BTX-ZW conjugates and 50% of DAH-HA-ZW hydrogels remained 3 weeks post-injection.
This bioimaging technique using invisible NIR fluorescence light can be exploited for various biomedical applications.
注射肉毒杆菌毒素（BTX）以减少面部皱纹是最常施行的整形手术之一。生物相容性水凝胶与BTX一起注射用于有效的组织增大。然而，难以确定有效组织增大的注射间隔。
用两性离子（ZW）近红外（NIR）荧光团标记BTX和透明质酸盐（HA）水凝胶，并在注射到BALB/c裸鼠后观察3周。
通过常规的EDC/NHS化学方法成功制备了BTX-ZW缀合物和二氨基己烷（DAH）-HA-ZW水凝胶。使用近红外荧光成像，我们证实注射3周后约10%的BTX-ZW缀合物和50%的DAH-HA-ZW水凝胶仍然存在。
这种使用不可见近红外荧光的生物成像技术可用于各种生物医学应用。
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