Schreier E, Ladhoff A M, Stompor S, Michel S
Department of Molecular Biology, Central Institute of Hygiene, Microbiology and Epidemiology, Berlin, G.D.R.
Acta Virol. 1988 Sep;32(5):403-8.
Immunogold labelling and in vitro transcription of influenza virus vRNA have been used to analyse the interaction of anti-influenza polymerase antibodies with influenza-ribonucleoprotein (RNP) complexes. The polymerase proteins (P proteins) were localized exclusively at one end of the RNP segments. In the course of transcription the amount of P protein decreased significantly. The in vitro transcriptase activity y of influenza A virus RNP complexes in the presence of anti-polymerase antibodies to the strain A/PR/8/34 was inhibited by 60%. In contrast, RNP transcriptase activity of influenza B virus was not inhibited by these antibodies.
免疫金标记和流感病毒vRNA的体外转录已被用于分析抗流感病毒聚合酶抗体与流感核糖核蛋白（RNP）复合物的相互作用。聚合酶蛋白（P蛋白）仅定位在RNP片段的一端。在转录过程中，P蛋白的量显著减少。在存在针对A/PR/8/34株的抗聚合酶抗体的情况下，甲型流感病毒RNP复合物的体外转录酶活性y被抑制了60%。相比之下，这些抗体并未抑制乙型流感病毒的RNP转录酶活性。
