Schreier E, Petzold D R, Michel S, Dittmann S
Department of Molecular Biology, Central Institute of Hygiene, Microbiology, Berlin, German Democratic Republic.
Arch Virol. 1988;103(3-4):179-87. doi: 10.1007/BF01311091.
The complete nucleotide sequence of the PB2 gene of influenza virus A/Chile/1/83 (H1 N1) is presented. Sequence comparison between A/Chile PB2 protein and the known PB2 sequences of the influenza strains A/WSN/33 (H1 N1), A/PR/8/34 (H1 N1), A/NT/60/68 (H3 N2), A/Kiev/59/79 (H1 N1), A/FPV/Rostock/34 (H7 N1), and B/Ann Arbor/1/66 indicates extensive amino acid homology for the influenza A virus PB2 proteins. Small clusters of basic amino acids are conserved in all PB2 proteins including the influenza B PB2 protein which has only 39% sequence homology overall to the PB2 polypeptides of type A influenza viruses. The evolutionary rate of 5.7 x 10(-3) nucleotide substitutions per site per year and 0.25% amino acid changes per year between the A/Chile/1/83 and A/NT/60/68 PB2 appears to be higher than that calculated earlier for A/NT, A/PR/8 and A/WSN. An unusually high degree of sequence change between A/Chile/1/83 and A/Kiev/59/79 PB2 polymerase was revealed and this is discussed in terms of its probable origin.
本文给出了甲型流感病毒A/智利/1/83（H1N1）PB2基因的完整核苷酸序列。将A/智利PB2蛋白与甲型流感病毒株A/WSN/33（H1N1）、A/PR/8/34（H1N1）、A/NT/60/68（H3N2）、A/基辅/59/79（H1N1）、A/禽痘病毒/罗斯托克/34（H7N1）以及乙型流感病毒B/安阿伯/1/66的已知PB2序列进行比较，结果表明甲型流感病毒PB2蛋白具有广泛的氨基酸同源性。在所有PB2蛋白中，包括与甲型流感病毒PB2多肽总体序列同源性仅为39%的乙型流感病毒PB2蛋白，都存在小的碱性氨基酸簇保守区。A/智利/1/83与A/NT/60/68 PB2之间每年每个位点的核苷酸替换率为5.7×10⁻³，每年的氨基酸变化率为0.25%，这似乎高于先前计算的A/NT、A/PR/8和A/WSN的相应值。A/智利/1/83与A/基辅/59/79 PB2聚合酶之间显示出异常高的序列变化程度，并从其可能的起源方面进行了讨论。
