Trujillo Carlos, Garcia-Sucerquia Jorge
Appl Opt. 2017 Jul 20;56(21):5877-5882. doi: 10.1364/AO.56.005877.
The cooperative execution of two metrics to automatically determine the best focal plane in digital lensless holographic microscopy (DLHM) is presented. This proposal is comprised of two stages: first, a quick coarse search over the whole reconstruction range by using Dubois's metric allows the finding of a range in which the best focal plane can be found. In a second stage, the modified enclosed energy (MEE) metric is used within the found range in the former stage to finely determine the best focal plane. While this cooperative implementation keeps the proven effectiveness of the MEE in DLHM, it reduces by at least 11 times the total computational complexity of the auto-focusing method with respect to the MEE method only. This proposal has been validated experimentally with DLHM holograms of a paramecium specimen, polystyrene beads, and the section of the head of a Drosophila melanogaster fly.
本文提出了在数字无透镜全息显微镜（DLHM）中协同执行两个指标以自动确定最佳焦平面的方法。该方案包括两个阶段：首先，通过使用杜波依斯指标在整个重建范围内进行快速粗略搜索，从而找到可以找到最佳焦平面的范围。在第二阶段，在前一阶段找到的范围内使用修正封闭能量（MEE）指标来精确确定最佳焦平面。虽然这种协同实现方式保留了MEE在DLHM中已被证明的有效性，但相对于仅使用MEE方法的自动聚焦方法，它将总计算复杂度降低了至少11倍。该方案已通过对草履虫标本、聚苯乙烯珠以及黑腹果蝇头部切片的DLHM全息图进行实验验证。
