Institute of Basic Theory, China Academy of Chinese Medical Sciences, Beijing 100700, China.
School of Pharmaceutical Science, Dalian University of Technology, Dalian 116024, China.
Nutrients. 2017 Oct 19;9(10):1146. doi: 10.3390/nu9101146.
Curcumin has been proven to have a weight-loss effect in a menopausal rat model induced by ovariectomy. However, the effects of curcumin on gut microfloral communities of ovariectomized (OVX) rats remains unclear. Here, we used high-throughput 16S rDNA sequencing to explore the effects of curcumin on microbial diversity in the gut of OVX rats. Female Wistar rats were subjected to either ovariectomy or a sham operation (SHAM group). The OVX rats were treated with vehicle (OVX group) or curcumin (CUR group) by oral gavage. After 12-week treatments, the weights of the bodies and uteri of rats were recorded, the levels of estradiol in the serum were assayed by electrochemiluminescence immunoassay (ECLIA). Then, the fragments encompassing V3-V4 16S rDNA hypervariable regions were PCR amplified from fecal samples, and the PCR products of V3-V4 were sequenced on an Illumina MiSeq for characterization of the gut microbiota. Our results showed that, compared to rats in the SHAM group, rats in the OVX group had more weight gain and lower levels of estradiol in the serum, and curcumin could cause significant weight loss in OVX rats but did not increase the levels of estradiol. Sequencing results revealed the presence of 1120, 1114, and 1119 operational taxonomic units (OTUs) found in the SHAM, OVX, and CUR groups, respectively. The percentage of shared OTUs was 86.1603%. Gut microbiota of rats from the SHAM or CUR group had higher levels of biodiversity and unevenness estimations than those from the OVX group. At the phyla level, compared to rats in SHAM group, rats in the OVX group had a higher ratio of phyla and in the gut; at the genus level, four differential gut microbiota (, , , and ) between SHAM and OVX groups were found, whereas seven differential gut microbiota (, , , , , , and ) between OVX and CUR groups were found. In conclusion, estrogen deficiency induced by ovariectomy caused changes in the distribution and structure of intestinal microflora in rats, and curcumin could partially reverse changes in the diversity of gut microbiota.
姜黄素已被证明可在去卵巢诱导的绝经后大鼠模型中具有减肥作用。然而，姜黄素对去卵巢（OVX）大鼠肠道微生物群落的影响尚不清楚。在这里，我们使用高通量 16S rDNA 测序来探索姜黄素对 OVX 大鼠肠道微生物多样性的影响。雌性 Wistar 大鼠接受去卵巢手术（OVX 组）或假手术（SHAM 组）。OVX 大鼠通过口服灌胃给予载体（OVX 组）或姜黄素（CUR 组）。经过 12 周的治疗，记录大鼠的体重和子宫重量，用电化学发光免疫分析法（ECLIA）测定血清中雌二醇的水平。然后，从粪便样本中扩增包含 V3-V4 16S rDNA 高变区的片段，并在 Illumina MiSeq 上对 V3-V4 的 PCR 产物进行测序，以表征肠道微生物群。我们的结果表明，与 SHAM 组大鼠相比，OVX 组大鼠体重增加更多，血清中雌二醇水平更低，而姜黄素可导致 OVX 大鼠显著体重减轻，但不会增加雌二醇水平。测序结果显示，SHAM、OVX 和 CUR 组分别存在 1120、1114 和 1119 个操作分类单元（OTU）。共享 OTU 的百分比为 86.1603%。来自 SHAM 或 CUR 组的大鼠的肠道微生物群具有更高的生物多样性和不均匀度估计值，而来自 OVX 组的大鼠则较低。在门水平上，与 SHAM 组大鼠相比，OVX 组大鼠肠道中的 和 比例更高；在属水平上，发现 SHAM 和 OVX 组之间有 4 种差异肠道微生物（ 、 、 和 ），而 OVX 和 CUR 组之间有 7 种差异肠道微生物（ 、 、 、 、 、 和 ）。总之，去卵巢引起的雌激素缺乏导致大鼠肠道微生物群的分布和结构发生变化，而姜黄素可以部分逆转肠道微生物群多样性的变化。
