Liang Qihui, Wu Di, Qiu Bailing, Han Nanyin
School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
Se Pu. 2017 Sep 8;35(9):918-926. doi: 10.3724/SP.J.1123.2017.05021.
Field-flow fractionation (FFF) is a kind of mature separation technologies in the field of bioanalysis, feasible of separating analytes with the differences of certain physical and chemical properties by the combination effects of two orthogonal force fields (flow field and external force field). Asymmetrical flow field-flow fractionation (AF4) is a vital subvariant of FFF, which applying a vertical flow field as the second dimension force field. The separation in AF4 opening channel is carried out by any composition carrier fluid, universally and effectively used in separation of bioparticles and biopolymers due to the non-invasivity feature. Herein, bio-analytes are held in bio-friendly environment and easily sterilized without using degrading carrier fluid which is conducive to maintain natural conformation. In this review, FFF and AF4 principles are briefly described, and some classical and emerging applications and developments in the bioanalytical fields are concisely introduced and tabled. Also, special focus is given to the hyphenation of AF4 with highly specific, sensitive detection technologies.
场流分级（FFF）是生物分析领域一种成熟的分离技术，通过两个正交力场（流场和外力场）的联合作用，能够分离具有某些物理和化学性质差异的分析物。非对称流场-流分级（AF4）是FFF的一个重要子变体，它将垂直流场用作第二维力场。AF4开放通道中的分离由任何组成的载液进行，由于其非侵入性特点，在生物颗粒和生物聚合物的分离中得到广泛且有效的应用。在此，生物分析物处于生物友好环境中，无需使用降解载液即可轻松灭菌，这有利于维持其天然构象。在本综述中，简要描述了FFF和AF4原理，并简要介绍和列出了生物分析领域的一些经典和新兴应用及发展情况。此外，特别关注了AF4与高特异性、高灵敏度检测技术的联用。
