不对称流场流分离技术在生物聚合物和生物颗粒的分离与特性分析中的应用。

Asymmetrical flow field-flow fractionation technique for separation and characterization of biopolymers and bioparticles.

机构信息

Laboratory of Analytical Chemistry, Department of Chemistry, P.O. Box 55, FIN-00014, University of Helsinki, Finland.

出版信息

J Chromatogr A. 2011 Jul 8;1218(27):4104-16. doi: 10.1016/j.chroma.2010.12.110. Epub 2011 Jan 18.

DOI:10.1016/j.chroma.2010.12.110

Abstract

Field-flow fractionation (FFF) is one of the most versatile separation techniques in the field of analytical separation sciences, capable of separating macromolecules in the range 10³-10¹⁵ g mol⁻¹ and/or particles with 1 nm-100 μm in diameter. The most universal and most frequently used FFF technique, flow FFF, includes three types of techniques, namely symmetrical flow FFF, hollow fiber flow FFF, and asymmetrical flow FFF which is most established variant among them. This review provides a brief look at the theoretical background of analyte retention and separation efficiency in FFF, followed by a comprehensive overview of the current status of asymmetrical flow FFF with selected applications in the field of biopolymers and bioparticles.

摘要

场流分离（FFF）是分析分离科学领域中最通用的分离技术之一，能够分离范围在 10³-10¹⁵ g mol⁻¹ 的大分子和/或直径为 1nm-100μm 的颗粒。最通用和最常用的 FFF 技术，即流动 FFF，包括三种技术，即对称流 FFF、中空纤维流 FFF 和不对称流 FFF，其中不对称流 FFF 是最成熟的变体。本综述简要介绍了 FFF 中分析物保留和分离效率的理论背景，然后全面概述了不对称流 FFF 的现状，并选择了在生物聚合物和生物颗粒领域的应用。