Zhao Zhenli, Li Yongsheng, Liu Haifang, Zhai Xiaoqiao, Deng Minjie, Dong Yanpeng, Fan Guoqiang
Institute of Paulownia, Henan Agricultural University, Zhengzhou, Henan, China.
Forestry Academy of Henan, Zhengzhou, Henan, P.R. China.
PLoS One. 2017 Oct 19;12(10):e0185455. doi: 10.1371/journal.pone.0185455. eCollection 2017.
Paulownia tomentosa is a fast-growing tree species with multiple uses. It is grown worldwide, but is native to China, where it is widely cultivated in saline regions. We previously confirmed that autotetraploid P. tomentosa plants are more stress-tolerant than the diploid plants. However, the molecular mechanism underlying P. tomentosa salinity tolerance has not been fully characterized. Using the complete Paulownia fortunei genome as a reference, we applied next-generation RNA-sequencing technology to analyze the effects of salt stress on diploid and autotetraploid P. tomentosa plants. We generated 175 million clean reads and identified 15,873 differentially expressed genes (DEGs) from four P. tomentosa libraries (two diploid and two autotetraploid). Functional annotations of the differentially expressed genes using the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes databases revealed that plant hormone signal transduction and photosynthetic activities are vital for plant responses to high-salt conditions. We also identified several transcription factors, including members of the AP2/EREBP, bHLH, MYB, and NAC families. Quantitative real-time PCR analysis validated the expression patterns of eight differentially expressed genes. Our findings and the generated transcriptome data may help to accelerate the genetic improvement of cultivated P. tomentosa and other plant species for enhanced growth in saline soils.
毛泡桐是一种生长迅速且用途广泛的树种。它在全球范围内种植，但原产于中国，在中国盐碱地区广泛栽培。我们之前证实，同源四倍体毛泡桐植株比二倍体植株更耐胁迫。然而，毛泡桐耐盐性的分子机制尚未完全阐明。以完整的白花泡桐基因组作为参考，我们应用新一代RNA测序技术分析盐胁迫对二倍体和同源四倍体毛泡桐植株的影响。我们生成了1.75亿条clean reads，并从四个毛泡桐文库（两个二倍体和两个同源四倍体）中鉴定出15873个差异表达基因（DEG）。使用基因本体论和京都基因与基因组百科全书数据库对差异表达基因进行功能注释，结果表明植物激素信号转导和光合作用活动对于植物应对高盐条件至关重要。我们还鉴定出了几个转录因子，包括AP2/EREBP、bHLH、MYB和NAC家族的成员。实时定量PCR分析验证了八个差异表达基因的表达模式。我们的研究结果以及生成的转录组数据可能有助于加速栽培毛泡桐和其他植物物种的遗传改良，以促进其在盐碱土壤中的生长。
