Shao Qianwen, Xu Jing, Deng Rong, Wei Wei, Zhou Bing, Yue Chao, Zhu Miaoling, Huang Xingen, Zhu Haitao
Department of Oncology, Jiangsu Province Hospital, The first Affiliated Hospital of Nanjing Medical University, Nanjing 210009, Jiangsu Province, PR China.
Genetic Testing Center, Department of Oncology of the First Affiliated Hospital, Nanjing Medical University, Nanjing, 210029, Jiangsu Province, PR China.
Biochem Biophys Res Commun. 2018 Jan 1;495(1):539-545. doi: 10.1016/j.bbrc.2017.10.076. Epub 2017 Oct 16.
Colorectal cancer (CRC) is one of the most frequent cancer in numerous of countries worldwidely. The initiation and progression of CRC is an extremely complex process, and have been suggested a correlation with Long non-coding RNAs (lncRNAs). Our results showed that lncRNA-422(ENST00000415820) significantly downregulated in the tissues and serum of CRC patients, and is closely associated with the poor prognosis. Then gain or loss of lncRNA-422 models in SW480 and SW620cells were established. The results showed that lncRNA-422 overexpression inhibited cell proliferation, migration, and invasion. Knockdown of lncRNA-422 promoted tumorigensis. Western blot and qRT-PCR were performed to examine the activity of the PI3K/AKT/mTOR pathway in CRC cells after alternation of lncRNA-422. Results showed that lncRNA-422 acts as a tumor suppressor by PI3K/AKT/mTOR pathway in CRC.
结直肠癌（CRC）是世界上许多国家中最常见的癌症之一。CRC的发生和发展是一个极其复杂的过程，并且已经有人提出其与长链非编码RNA（lncRNAs）存在关联。我们的结果显示，lncRNA-422（ENST00000415820）在CRC患者的组织和血清中显著下调，并且与不良预后密切相关。随后在SW480和SW620细胞中建立了lncRNA-422的过表达或敲低模型。结果显示，lncRNA-422的过表达抑制了细胞增殖、迁移和侵袭。lncRNA-422的敲低促进了肿瘤发生。在lncRNA-422改变后，进行蛋白质免疫印迹法（Western blot）和实时定量聚合酶链反应（qRT-PCR）以检测CRC细胞中PI3K/AKT/mTOR信号通路的活性。结果显示，lncRNA-422在CRC中通过PI3K/AKT/mTOR信号通路发挥肿瘤抑制作用。
