Cheng C L, Dewdney J, Nam H G, den Boer B G, Goodman H M
Department of Genetics, Harvard Medical School, Boston, MA.
EMBO J. 1988 Nov;7(11):3309-14. doi: 10.1002/j.1460-2075.1988.tb03201.x.
We have isolated two nitrate reductase genes and their corresponding cDNAs from Arabidopsis thaliana. Sequences of the two cDNAs, when compared to a sequence of a barley cDNA clone, confirm their identity as nitrate reductase clones and show that they are closely related. The two genes have been mapped using restriction fragment length polymorphisms; gNR2 is close to the previously identified chl-3 locus and is probably identical to it, while gNR1 maps to a new locus (NIA1) on chromosome 1, near gl-2.
我们从拟南芥中分离出了两个硝酸还原酶基因及其相应的cDNA。将这两个cDNA的序列与一个大麦cDNA克隆的序列进行比较后，证实了它们作为硝酸还原酶克隆的身份，并表明它们密切相关。利用限制性片段长度多态性对这两个基因进行了定位；gNR2靠近先前鉴定的chl-3位点，可能与之相同，而gNR1定位于第1号染色体上靠近gl-2的一个新位点（NIA1）。
